蛇毒NGF对PC12细胞MAPK表达的影响

Effect of Nerve Growth Factor from the Venom of A.halys on the Expression of Mitogen-activated Protein Kinases of PC12 Cells.

有丝分裂原激活的蛋白激酶 (MAPK)是磷酸化瀑布中涉及的一类重要蛋白激酶 .阐明各种胞外因素对MAPK的调节 ,以及各种外界信息所经历的具体过程是摆在研究者面前的挑战性任务 .用含有长白白眉蝮蛇(A .halys)蛇毒神经生长因子 (NGF)的培养基培养PC12细胞 ,观察MAPK和MAPK激酶 (MAPKK)表达及MAPK的活性 .结果表明 ,A .halys蛇毒NGF使PC12细胞的MAPK和MAPKK的表达及MAPK活性明显增加 .当蛇毒NGF浓度在 2 5～ 10 0 μg/L时 ,随浓度的增加 ,酶的表达和活性呈线性增加 .由A .halys蛇毒NGF诱导的MAPK和MAPKK表达的增加依赖于蛋白激酶C (PKC)

Mitogen activated protein kinase (MAPK) is a group of important protein kinases involved in phosphorylation cascade in the mitogen initiated signal transduction pathways.NGF from Agkistrodon halys has been used to investigate its effects on MAPK and MAPKK of PC12 cells. The results showed that this kind of NGF increased MAPK and MAPKK expression and MAPK activity. The above increases were NGF concentration dependent in the range of 25～100 μg/L. The use of PKC inhibitor H 7 indicated that the increased expressions of MAPK and MAPKK expression were PKC dependent .