利用cDNARDA技术研究BXSB狼疮小鼠差异表达的基因

Isolation of the Differentially Expressed Genes in the BXSB Mouse Model of Systemic Lupus Erythematosus by cDNA-RDA Technique.

利用近年来发展起来的代表性差异分析cDNA RDA (cDNA representationaldifferenceanalysis)技术筛选BXSB红斑狼疮小鼠发病相关基因 .发现了 3个新的表达序列标签 (EST)片段 ,在GenBank中的登录号分别为AF0 60 113 ,AF0 60 111,AF0 60 110 ,同时发现了一些已知与自身免疫病相关的基因如逆转录病毒衣壳蛋白、Line 1逆转录酶等 .通过RDA技术可能发现系统性红斑狼疮发病相关新基因 。

In order to isolate the related genes in the development of systemic lupus erythematosus (SLE) in BXSB mice, bone marrow cells were seperated from the BXSB and C57 BL 6 mouse, poly(A) RNA was extracted, the cDNAs were synthesized by reverse transcription, and the differentially expressed genes were cloned by cDNA representational difference analysis(RDA). Three novel genes were isolated, their accession numbers in GenBank are AF060113, AF060111, AF060110; at the same time, some genes that have been reported to be related to the development of SLE such as endogenous retrovirus and Line 1 reverse transcriptase were found. This can provide a novel method to do further research about autoimmune diseases.