摘要
目的探讨大黄素对小鼠结肠癌细胞CT26.WT的抑制作用。方法 MTT法检测大黄素对结肠癌细胞CT26.WT增殖的抑制作用;细胞划痕实验检测大黄素对结肠癌细胞CT26.WT迁徙的抑制作用;流式细胞仪检测大黄素对结肠癌细胞CT26.WT表达CC族趋化因子受体4(CCR4)和叉头蛋白3(Foxp3)的影响。结果大黄素明显抑制CT26.WT细胞的增殖,加入的大黄素浓度越高抑制效果越好(P<0.05);大黄素抑制CT26.WT细胞的迁徙,抑制效果随浓度升高而愈加明显(P<0.01);大黄素抑制CT26.WT细胞的Foxp3表达,而表达CCR4的肿瘤细胞的比例同样低于不加药组,这种抑制作用与大黄素的药物浓度呈负相关(P<0.01)。结论大黄素抑制小鼠结肠癌的CT26.WT细胞增殖,Foxp3和CCR4在CT26.WT细胞的表达降低,可能是大黄素抑制其迁徙的原故。
Objective To investigate the mechanism of emodin in inhibiting of CT26.WT cell line.Methods The proliferation and migration capacities of CT26.WT cells were assessed by MTT method and scratch assay,respectively;while the expression of CCR4 and Foxp3 was assessed by flow cytometry.Results Significant inhibition of cell proliferation and migration of CT26.WT was observed by Emodin in a concentration-dependent manner（P 0.05）;so was the down-regulation of Foxp3 and CCR4（P 0.01）.Conclusion Emodin inhibits the CT26.WT cell proliferation and migration,which is probably via the down-regulation of CCR4 and Foxp3.
出处
《广东医学》
CAS
CSCD
北大核心
2013年第12期1797-1800,共4页
Guangdong Medical Journal
基金
广东省中医药局建设中医药强省立项资助科研课题(编号:20121104)
