莪术醇对乳腺癌细胞MDA-MB-231侵袭能力的影响

Effects of curcumol on the invasiveness capability of breast cancer cell line MDA-MB-231

目的探讨莪术醇对乳腺癌MDA-MB-231细胞增殖能力、侵袭能力的影响。方法利用MTT实验检测乳腺癌MDA-MB-231细胞的增殖能力;应用基质胶侵袭小室实验检测乳腺癌MDA-MB-231细胞的侵袭能力;采用real-time PCR法检测经不同浓度的莪术醇药物干预的MDA-MB-231细胞,观察羟基末端结合蛋白1(CtBP1)在不同组中表达的变化。结果不同浓度的莪术醇(100、50、25、12.5μg/mL)处理下,细胞数量呈差异性、阶梯状下降,浓度为100μg/mL的细胞数量减少最为明显,差异有统计学意义(P<0.05)。同时细胞侵袭能力按药物浓度递减而减弱,差异有统计学意义(P<0.05)。Real-time PCR的结果显示,莪术醇可显著下调MDA-MB-231中CtBP1的表达(P=0.000)。结论莪术醇对乳腺癌MDA-MB-231细胞增殖有明显的抑制作用,对其侵袭能力亦有较强影响,药物浓度越高抑制增殖、侵袭、下调CtBP1表达的效果越明显。

Objective To evaluate the effects of curcumol on multiplication and invasiveness capacities of breast cancer cell MDA-MB-231.Methods The multiplication and invasiveness capacities of MDA-MB-231 cells were assessed with MTT assay and transwell test,respectively;while the expression of CtBP1 in different concentrations of curcumol was evaluated with real-time PCR.Results Significant inhibition on the multiplication and invasiveness capacities of MDA-MB-231 cells were revealed by curcumol on concentration-dependent manner（P 0.05）.Meanwhile,significant down-regulation of CtBP1 was observed by curcumol in MDA-MB-231 cells（P = 0.000）.Conclusion Curcumol can inhibit the proliferation of MDA-MB-231 cells,induce apoptosis,impact the invasive ability,and down regulate the expression of CtBP1 in concentration-dependent manners.