应用B.t.和SBTi基因提高水稻抗虫性的研究

Research on Improving Rice Resistance to the Pest by B.t. and SBTi Genes

利用基因枪法将单个B .t.基因或与SBTi基因一起导入到两个华南地区优良籼稻品种中 ,获得 2 1个转B .t.单基因的植株系 ,4个转B .t.和SBTi双基因的植株系。对R1代植株的分子杂交和遗传分析表明 ,3个转双基因系中多个拷贝的B .t.和SBTi基因均是整合在植株基因组同一染色体上相同或相近位点。Northernblot证明在R2 代转基因植株中B .t .基因稳定表达。对稻纵卷叶螟的抗性实验表明 ,转B .t .单基因或B .t.和SBTi双基因的转基因植株均较原种对照有更强的抗性 ,而转双基因植株较转单基因植株又有更强的抗性。

B.t.gene alone or and SBTi gene together were introduced into two elite indica rice varieties grown in South China by bombardment. And 21 independent transgenic lines containing B.t. gene and 4 independent transgenic lines containing B.t. gene and SBTi gene were obtained. Molecular and genetics analysis for R 1 plants showed integration of multiple transgenes occurred at one genetic locus. Northern blot result proved B.t. gene expressed in R 2 transgenic plants stably. Bioassays using R 2 transgenic plants with leaffolder ( Cnaphalocrosis medinalis) ,indicated that transgenic rice plants are more resistant to the pest than untransformed control plants. And those transgenic plants containing B.t. and SBTi genes showed more resistance compared with those plants containing B.t. gene.