手性苯基环氧乙烷的生物不对称合成

Asymmetric Biosynthesis of Chiral Styrene Oxide

以苯乙烯为唯一碳源和能源 ,从不同来源的土壤样品中初筛分离出 12株好氧细菌和 2株真菌 ,经复筛 ,对液体培养物进行手性气相色谱分析 ,得到一株产生手性苯基环氧乙烷活力较高的菌种PS 12 0 6 ,并对其发酵、产酶及苯乙烯的全细胞转化进行了研究 ,利用微生物细胞在 30℃ ,pH 7 0 ,10mmol/L磷酸缓冲液中转化 0 5 %苯乙烯 10h ,获得 (R) 苯基环氧乙烷 ,e .e%值为 80 % ,转化产率为 35 %。

Used styrene as the sole carbon and energy source,12 strains of aerobic bacterial and two strains of fungi were screened from a series soil samples.These strains were able to convert styrene to styrene oxide.HPLC and chiral GC method were used for determination the substrate,product and e.e value of culture broth.There were obvious effect on the biomass and epoxidase activity under various conditions in the fermentation.Styrene epoxidation of the strain PS 1206 was studied in different reaction time.35% of conversion yield and 80% e.e value of (R)\|styrene oxide could been obtained at the optimum conditions.