重组人血清白蛋白发酵过程生长期的代谢计算

Metabolic Calculation of the Growth Phase in rHSA Fermentation

结合元素平衡和代谢平衡 ,建立了重组人血清白蛋白发酵过程生长期的数学模型 ,并按单纯形多变量最优化方法估算了模型的未知参数。该模型能较好地描述重组人血清白蛋白发酵过程生长期中各宏观反应速率之间的关系 ,为重组人血清白蛋白宿主菌—Pichiapastoris的高密度培养提供了依据。

The model equations of the growth phase of rHSA fermentation were derived on the base of both elemental balance and metabolic balance.The unknown parameters of the model were estimated by multivariable optimization.The model can preferably describe the relations between different macroscopic reaction rates of the process and provide the key for the high\|density cultivation of Pichia pastoris.