摘要
The eukaryotic expression plasmid (pcDNA3 CEA)containing with the gene coding for the Carcinoembryonic antigen(CEA) had been gotten with RT PCR and gene recombination techniques.Using enzymolysis,ligation and other techniques,an eukaryotic coexpression plasmid(pcDNA3 CEA/HN)containing two expression unites of CEA and Newcastle disease virus HN gene that may have the function of immunoenhancement had been constructed.The plasmid will lay a foundation for further researching CEA nucleiotide vaccine,adjuvant and their effect of special antitumor immune.
The eukaryotic expression plasmid (pcDNA3 CEA)containing with the gene coding for the Carcinoembryonic antigen(CEA) had been gotten with RT PCR and gene recombination techniques.Using enzymolysis,ligation and other techniques,an eukaryotic coexpression plasmid(pcDNA3 CEA/HN)containing two expression unites of CEA and Newcastle disease virus HN gene that may have the function of immunoenhancement had been constructed.The plasmid will lay a foundation for further researching CEA nucleiotide vaccine,adjuvant and their effect of special antitumor immune.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第5期641-644,共4页
Chinese Journal of Biotechnology
关键词
癌胚抗原
新城疫病毒HN-基因
共表达质粒
疫苗
Carcinoembryonic antigen,newcastle disease virus HN gene,coexpression plasmid
