黄花倒水莲(Polygala fallax Hemsl.)对^(60)Coγ射线辐射损伤小鼠组织DNA的防护作用

Protective Effects of Polygala fallax Hemsl1 on ^(60)Coγ Irradiated-induced DNA Damage in Mice

目的:探讨黄花倒水莲对辐射损伤小鼠组织DNA的防护作用.方法:实验设计分照射前给药和照射后给药2个模块,每模块各5组.复制60Coγ射线(辐射强度为6Gy)对雄性昆明种小鼠辐射损伤的病理模型,黄花倒水莲提取液2、4、8 g/(kg.d)连续灌胃7天,正常对照组、照射模型组给予等量生理盐水,用彗星试验检测小鼠多种组织细胞DNA损伤情况.结果:黄花倒水莲照射前给药和照射后给药,均可明显降低小鼠肺、肝、脾、睾丸、淋巴细胞DNA的尾部DNA百分率和尾矩(P<0.01),呈一定的量效关系.结论:黄花倒水莲可以减轻60Coγ射线所致小鼠组织DNA的损伤,具有辐射防护作用.

Objective： To explore the protective effects of Polygala fallax Hemsl1for DNA of 60Coγ-ray irradiated mice.Methods： The mice model of radiation damage was established by single whole body at 6Gy 60 Coγ.The experiments include two modules： medication before radiation and after radiation.Each module includes lowdose,medium-dose and high-dose groups plus irradiated which was given extracting solution of Polygala fallax Hemsll at 2、4、8 g/（kg.d）for 7 consecutive days.The negative group and irradiation group were given normal saline.DNA damage was detected by single cell gel electrophoresis.Results： Tail DNA% and tail moment of tissue cells of the given Polygala fallax Hemsll before radiation and after radiation groups were reduced（P0.01）,presenting a relation of dosage-effect.Conclusions： Polygala fallax Hemsll can reduce the DNA damage induced by 60 Coγ irradiation in mice and has protective effects for radiation.