坡地放养成年三黄鸡肠道正常菌群的研究

Study on Normal Flora in the Intestinal Canal of Adult Sanhuang Chicken

【目的】研究肠道不同部位的pH值和正常菌群分布数量的规律以及肠道微生态平衡与禽类健康生长的关系.【方法】将肠道内容物样品稀释涂布于选择性培养平板进行目的菌计数;采用精密PH试纸测定肠道pH值和烛缸二氧化碳培养法培养厌氧菌.【结果】肠道中厌氧菌双歧杆菌(1010.1cfu/g～1011.51cfu/g)和乳酸杆菌(109.1cfu/g～109.7cfu/g)为优势菌群,需氧菌(107.2cfu/g～109.5cfu/g)在肠道内容物移动过程中有不同规律的变化,但厌氧菌和需氧菌在消化道中总体呈两头少中间多的分布规律.【结论】肠道pH值与微生物分布数量存在一定的关系;需氧菌、厌氧菌及各兼性菌在一定的环境中相互作用,得以共同生长,体现出不同的数量分布规律.

Objective： To study the pH value of different parts of intestine,the law of normal flora’s distribution and how the balance of intestinal micro-flora affects poultry’s growth.Methods： Count the number of objected bacteria from the diluted samples of intestinal contents daubed on the selective culture plates;determine the intestinal pH value by using precise pH test paper;cultivate anaerobic bacteria with candle cylinder carbon dioxide culture method.Results： Bifidobacterium（1010.1cfu/g～1011.51cfu/g） and lactobacillus（109.1cfu/g～109.7cfu/g） are the dominant bacteria in the intestine;the distribution of aerobic bacteria in intestine presents a irregular change law（107.2cfu/g～109.5cfu/g） during the moving process of intestinal contents,while the distribution of anaerobic bacteria and aerobic bacteria in the digestive tract presents a regular change of ＂fewer in both ends and more in the middle＂.Conclusion： There is a certain relationship between the intestinal pH value and the distribution of microorganism;aerobic bacteria,anaerobic bacteria and facultative bacteria interact and grow together in a certain environment,reflecting different distribution laws;further researches are needed on the macro and micro environment,and their relation with chicken organism.