HIV-1 Gag p^(17-)p^(24)在痘苗病毒中的表达及性质研究

Expression and Characterization of HIV-1 Gag p^(17-)p^(24) Protein

研究了重组痘苗病毒表达的HIV1核心蛋白(Gag)p17p24蛋白的一些生物学及免疫学特点。间接免疫荧光、DotELISA及Westernblot结果表明,构建的两株重组病毒分别表达了HIV1Gagp24及p17p24融合蛋白。电镜观察证实,Gagp24及p1724重组蛋白均可形成病毒样粒子。重组病毒可诱导小鼠产生抗HIV1Gagp24抗体。重组病毒感染BHK21细胞后,可见由于细胞凋亡而致的染色体DNA断裂“梯子”电泳图。

The biological and immunological characteristics of HIV 1 core protein p17 24 expressed by recombinant vaccinia viruses were studied.The results of indirect immunofluorescence assay (I IFA),Western blot and dot ELISA showed that the two recombinants could express the p24 gag and p17 24 gag fusion proteins in infected cell lines respectively.The electromicroscope observation revealed that the expressed proteins could also assemble into virus like particles.The recombinant vaccinia viruses can also stimulate mice for the formation of anti HIV 1 Gag p24 antibody.When infected with the recombinant viruses,the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed.