人TRAIL基因cDNA的克隆及其在COS-7细胞中的表达

Cloning of Human TRAIL cDNA and Its Expression in COS-7 Cells

ＴＲＡＩＬ(ＴＮＦｒｅｌａｔｅｄａｐｏｐｔｏｓｉｓｉｎｄｕｃｉｎｇｌｉｇａｎｄ)是最近克隆的肿瘤坏死因子(ＴＮＦ)家族的新成员,由于它的蛋白质结构和生物学效应类似于ＦＡＳ/ＡＰＯ1Ｌ,因此,也被称为ＡＰＯ2Ｌ。在低浓度下,ＴＲＡＩＬ能迅速地诱导多种肿瘤细胞系的?..

The human TRAIL cDNA was amplified with the total RNA from the human acute promyelocytic leukemia cell line HL 60 by means of RT PCR,and was cloned into the pGEM T vector.The DNA sequence analysis showed that it was consistent with the published sequence.Then,the insert of human TRAIL cDNA was subcloned into the mammalian expression vector pcDNA3.The hybrid plasmid pcDNA3 hTRAIL was transformed into COS 7 cells,and transiently expressed in the COS 7 cells.The activity of the expressed product could induce apoptosis in U937 cell line.