正交设计优化大孔树脂富集、纯化地肤子总皂苷的工艺

Orthgonal design optimization on purification process of total saponin from Kochia scoparia with macroporous resin D101

目的优化D101大孔吸附树脂纯化地肤子总皂苷的工艺条件及参数。方法以总皂苷和地肤子皂苷Ic含量为指标,采用正交试验方法考察上样量、径高比、洗脱溶剂用量和洗脱速度4个因素对纯化效果的影响。结果经优化的纯化工艺条件为:洗脱溶剂用量(mL)与上样量(mL)比为4∶1,洗脱速度为1mL.min-1,湿树脂量(g)与上样量(mL)比为2∶1,径高比为1∶8。结论经该工艺纯化后明显提高了总皂苷的纯度和含量。

OBJECTIVE To optimize the purification process of total saponin from Kochia scoparia with macroporous resin D101. METHODS Based on orthogonal design,the contents of total saponin and the contents of Momordin Ic worked as indexes, the factors such as adsorption capacity, the column diameter-height ratio, eluant volume and elution velocity were investigated. RESULTS The optimum elution parameters were as foUows：the eluant volume of 50% alcohol-the sample was 4：1 ,The wet resin amount （g） -the sample volume （mL） was 2： 1, the column diameter-height ratio was 1：8, the elution velocity was 1 mL ·min^- 1, respectively. CONCLUSION The purification process can significantly improve the purity degree and content.