摘要
目的建立高效液相色谱法(HPLC)测定小鼠胰腺组织中奥硝唑浓度方法。方法以DISCOVERY ODS C18柱(5μm,250mm×4.6mm)为色谱柱,甲醇-0.01mol.L-1磷酸二氢钾溶液=22∶78(v/v)为流动相,流速1.0mL.min-1,柱温30℃,检测波长310nm,组织样品用三氯乙酸沉淀蛋白。结果小鼠胰腺组织中的内源物质不干扰奥硝唑的测定,奥硝唑在0.1～200μg.mL-1浓度范围内峰面积与浓度线性关系良好(r=0.999 9),最低检测限为0.1μg.mL-1。平均回收率为(98.26±1.18)%,高、中、低3种浓度的日内差(RSD)<5%,日间差(RSD)<10%。结论本方法简便、快速、准确,重现性好,可应用于奥硝唑组织内药动学研究。
OBJECTIVE To establish a reversed-phase HPLC （RP-HPLC） technique for the determination of the omidazole （ONZ） concentration in pancreatic tissue. METHODS The following RP-HPLC conditions were set up：DISCOVERY ODS Cls column（5 μm,250mm×4.6mm） as stationary phase;a mixture of methanol and 0. 01mol·L^-1 potassium dihydrogen phosphate （22：78） at a flow rate of 1.0mL ·min^-1 as mobile phase;UV detection at 310nm. The samples were deproteinised with trichloroacetic acid. RESULTS ONZ concentration in pancreatic tissue showed a linear plot in the range of 0. 1 - 200μg·mL^-1（ r = 0. 999 9）. The minimal quantitative eoncentration was 0. 1μg·mL^-1. The mean reeovery at the quantitative concentrations of ONZ was （98.26 ±1.18 ）%. The coefficients of variation for within-day performance were found to be less than 5%, and between-day performance were less than 10%. CONCLUSION The RP-HPLC is simple and sensitive to quantify omidazole concentration in pancreat- ic tissue, and may be used to monitor the PK parameters in tissues.
出处
《海峡药学》
2013年第6期21-23,共3页
Strait Pharmaceutical Journal
基金
常州市科技局指导性项目(常州四药临床药学研究基金资助项目)(基金编号:CY20119022)
