低温胁迫对红麻细胞质雄性不育系及其保持系的形态及光合生理的影响

Effects of low temperature on morphology and photosynthetic physiologic of CMS and maintain lines of kenaf

以7对红麻细胞质雄性不育系及其保持系幼苗为材料,在12℃/6℃(昼/夜)低温胁迫15d,测定植株形态、光合作用相关参数以及叶绿素含量变化。结果显示:1)持续低温胁迫下,植株生长缓慢,不育系的相对茎粗和叶面积增加量高于保持系。2)随着胁迫时间的延长,叶片的净光合速率和叶绿素(Chl)总量持续降低,气孔导度的变化表现为先下降后上升再下降,而气孔限制值Ls和Chl a/Chl b的变化则是先上升后下降。3)利用主成分分析和隶属函数相结合的方法,得出红麻细胞质雄性不育系的耐冷性高于保持系,细胞质雄性不育系/保持系耐冷性较强的组配是F3A/B、917A/B和722A/B,本研究结果对于红麻越冬栽培和耐低温品种选育有重要的参考价值。

Seven groups of CMS and maintain lines of kenaf leaves had been used to investigate the changes of plant＇s morphological feature,some parameters related to photosynthesis and content of chlorophyll under low temperature stress at 12℃/6℃（day and night）for 15 days.The results revealed that：1） The kenaf plant grew slowly,and the increase of stem diameter and leave area in CMS were greater than those from the maintain lines.2） Along with the increase of low temperature stress,the Pn and the total chlorophyll content were decreased;the Gs showed low-high-low changes,whereas the Ls and the ratio of Chl a/Chl b increased firstly and then decreased.3） Principal Component Analysis and membership function had been used to analyze in this research It was showed that CMS were more tolerant to low temperature stress than their maintain lines;F3A/B,917A/B and 722A/B were the three strongest groups.The research also provided a valuable suggestion on winter cultivation and cold resistance breeding of kenaf.