桂东南地区芋疫霉的交配型研究

A Research on Mating Types of Phytophthora colocasiae in South-eastern Guangxi Province

芋疫病是广西芋头上的重要病害。本研究从桂东南地区部分乡镇的芋头产区采集并分离获得12个芋疫霉菌株,通过与辣椒疫霉A1交配型HD-3菌株和A2交配型S-5菌株对峙培养来测定其交配型,并以ITS1/ITS4通用引物对基因组DNA进行PCR扩增,得到各菌株的ITS序列。结果表明,通过对ITS序列的Blast比对,发现所有菌株的ITS序列与GenBank中登录的芋疫霉（Phytophthora coloc asiae）不同分离物序列同源性达97%~100%,证明所采样本均为芋疫霉。从桂东南地区采集到的12个芋疫霉菌株均为A2交配型,未发现其它交配型菌株。

Tar o leaf blight is an important disease in Guangxi province. Disease samples were collected from different regions in south-eastern Guangxi. A total of 12 isolates ofPhytophthora colocasiae were isolated from the infected taro leaves. Confront culture method was used to detect P. colocasiae mating types with P. capsici A1 （HD-3） and A2 （S-5） mating types. ITS sequences of different isolates were determined by sequencing PCR products generated with genomic DNA of the isolates as templates and universal ITS 1/ITS4 primers. Blast results revealed that the isolates shared 97%-100% sequence homology to isolates ofP. colocasiae in the GenBank. All 12 isolates ofP. colocasiae belonged to A2 mating type.