供体细胞、去核方法以及TSA处理对巴马小型猪手工克隆胚胎发育的影响

Influences of Different Donor Cells, Enucleation Methods and TSA Treatments on the in vitro Development of Handmade Cloned Embryos of Guangxi Bama Minipig

本文用不同的供体细胞、去核方法以及TSA处理浓度研究其对巴马小型猪手工克隆胚发育的影响,试验结果发现:克隆胚的发育率在相同代次的不同供体细胞之间,极体定向和化学辅助去核法之间均无显著性差异(p>0.05);TSA浓度为40nmol/L时克隆胚的囊胚率最高,但与对照组相比无显著性差异(p>0.05)。这说明三种供体细胞均能支持巴马小型猪克隆胚的早期发育,化学辅助和极体定向去核法均为较好的去核方法。

The cloned embryos were constructed with different donor cells, enucleation methods and then treat-ment with different concentration of TSA in order to evaluate their potential effects on the development of Guangxi Bama Minipig handmade cloned embryos in vitro. It was showed that the development rate of handmade cloned embryos were not only similar between the three groups of cell types with the same generation, but also be- tween the chemically assisted handmade enucleation （CAHE） and polar body-oriented-handmade enucleation （OHE） groups （p〉0.05）. Although the blastocyst rate in the 40 nmol/L TSA group was higher, there was no significant dif- ference among the control and other groups. It is concluded that the three kinds of donor cells could well support in vitro embryonic development ofBama minipig handmade cloned embryos, CAHE and OHE could both be the enucleation method for handmade cloning.