基于高通量测序的凡纳滨对虾的转录组分析

Deep Sequencing-based Transcriptome Analysis of Litopenaeus vannamei

凡纳滨对虾是世界养殖最广泛的甲壳动物,但是目前凡纳滨对虾的基因组及转录组数据还比较缺乏。为了获得凡纳滨对虾的转录组信息,本研究应用454高通量测序技术对凡纳滨对虾肝胰腺的转录组进行测序。获得了500177条凡纳滨对虾EST,平均长度363bp。拼接获得了20225条unigene,长度范围50~8980bp,平均长度507bp。所有unigene与NCBI的非冗余蛋白质数据库（Nr）进行相似搜索（E值〈10-5）,结果一共有13676条unigene（68%）与数据库中的已知基因同源。此外,还对unigene进行了GO、COG和KEGG的功能注释、分类或通路分析。我们通过高通量测序,获得了丰富的凡纳滨对虾转录组信息,为凡纳滨对虾的新基因克隆和基因组学研究提供了有价值的数据。

Litopenaeus vannamei shrimp is the most extensively farmed crustacean species in the world. However, the genome and transcriptome data ofLitopenaeus vannamei is still relatively lacking. To obtain transcriptome infor-mation ofLitopenaeus vanname, the transcriptome ofLitopenaeus vannamei hepatopancreas was sequenced by 454 high-throughput sequencing technology. A total of 500 177 ESTs were produced, with an average length of 363 bp. De novo assembly of ESTs data generated 20 225 unigenes between 50 and 8 980 bp in length, with an average length of 507 bp. Similarity searches against the NCBI-Nr database revealed that 13 676 （68%） of these unigenes have significant matches （E-value〈10^-5）. In addition, the unigenes were similarity search against the GO, COG and KEGG databases, and were annotated with gene functional descriptions, gene ontology terms, or pathways. By high- throughput sequencing, we obtained abundant transcriptome information that could contribute to novel gene identi-fication and genome research in Litopenaeus vannamei.