红掌‘Fiesta’和‘Altimo’离体繁殖体系的建立

Establishment of in vitro Propagation System Based on Anthurium andraeanum (‘Fiesta’and‘Altimo’)

为了建立红掌新品种‘Fiesta’和‘Altimo’离体繁殖体系,本文以叶柄为试验材料,进行外植体消毒、愈伤诱导和植株再生试验。结果表明:最佳消毒方法为50mg/L制霉菌素浸泡20min、70%乙醇浸泡30s、0.1%升汞浸泡5min、无菌水清洗6次,该方法处理的外植体污染率和褐化率均较低;综合考虑出愈率和愈伤活力,‘Fiesta’和‘Altimo’的最佳愈伤诱导培养基分别为1/2MS+6-BA1.00mg/L+2,4-D0.2mg/L和1/2MS+6-BA2.00mg/L+2,4-D0.10mg/L;最适分化培养基均为1/2MS(蔗糖25.00g/L)+6-BA0.50mg/L+2ip0.10mg/L+NAA0.05mg/L。本研究建立了红掌品种‘Fiesta’和‘Altimo’的愈伤诱导和再生体系,结果为种苗快繁和遗传转化研究提供了技术参考。

To establish the in vitro propagation system of Anthurium andraeanum cv.‘Fiesta’and‘Altimo’, in this study, we employed petiole as explant, to optimize protocol of disinfection, callus induction, and plantlet regeneration in this study. The results showed that the best disinfection method was sequentially immersing for 20 min in 50 mg/L Nystatin, 30 s in 70%Ethanol, 5 min in 0.1%HgCl2, and then washing 6 times in sterile water.‘Fiesta’and‘Altimo’displayed the highest frequencies of callus induction on improved medium：1/2MS＋6-BA 1.00 mg/L＋2,4-D 0.2 mg/L and 1/2MS＋6-BA 2.00 mg/L＋2,4-D 0.10 mg/L respectively. For both cultivars, the optimum shoots induction medium were 1/2MS （25.00 g/L sucrose）＋6-BA 0.50 mg/L＋2ip 0.10 mg/L＋NAA 0.50 mg/L. This study has successfully outlined a rapid, high frequency in vitro propagation for Anthurium, it provide technical reference for Seedling Production and genetic transformation.