2种单核吡啶-铜配合物与DNA作用及抗癌活性研究

DNA-binding and anticancer properties of two mononuclear copper-pyridine complexes

合成了2种单核吡啶-铜配合物[Cu(Py2)Cl2](1)和[Cu(Py2)Br2](2).通过X射线单晶衍射、元素分析、红外光谱等对其进行了结构表征;运用黏度、紫外光谱和荧光光谱等测试方法研究了标题配合物与鲑鱼精DNA的作用方式;采用MTT法研究了配合物对人体癌细胞的增殖抑制作用.结果表明:2种配合物与DNA的作用模式均为插入方式;在物质的量的浓度为50μmol/L下,该配合物对5种肿瘤细胞HeLa,NCI-H460,MCF-7,HL-60和HepG-2都表现出一定的活性.

Two mononuclear copper-pyridine complexes [Cu（Py）2Cl2]（1） and [Cu（Py）2Br2]（2） were synthesized and their structures were characterized by X-ray single-crystal diffraction,elemental analysis and IR spectra.The interactions of the complexes with salmon sperm DNA were investigated by viscosity analysis,UV and fluorescence spectroscopic techniques.The experimental results indicated that the binding modes between the two complexes and DNA should be intercalation.At the same time,the anticancer experiments by MTT method indicated the complexes exhibit some activity against HeLa,NCI-H460,MCF-7,HL-60 and HepG-2.