蓝莓花色苷提取与纯化工艺的研究

Extraction and Purification Research of Anthocyanins of Blueberry

本实验主要对蓝莓花色苷的提取纯化工艺进行了研究。通过单因素分析,响应面优化实验得到蓝莓花色苷粗提物,比较5种大孔树脂对其吸附和解吸性能,选出最佳性能的树脂,并优化纯化条件。通过响应面实验得到最佳提取条件为:提取温度44℃,时间120min,料液比1∶24。并确定HP2MGL大孔树脂为纯化最佳树脂,最佳纯化条件为:动态吸附上样速率3mL/min,pH=2.0,70%乙醇作为洗脱剂,2mL/min流速,4柱体积乙醇可完全洗脱花色苷。经树脂纯化后蓝莓花色苷为紫黑色粉末,色价为58.96,回收率88.53%。

The paper main studied the extraction and purification of anthocyanins of blueberry. Through the single factor analysis and optimal response surface experiment to get blueberry anthoeyanins. 5 kinds of macroporous resins＇ adsorption and desorption performance were compared, selected the best performance, and optimization of the purifi-cation condition. The results showed that optimal extraction conditions were as following：extraction temperature was 44~C, time was 120rain, solid-liquid ratio was 1：24. And the optimal purification resin was HP2MGL macroporous resin, the optimal purification conditions were： The dynamic adsorption of sample rate 3mL/min, pH 2.0, eluant 70% ethanol, velocity 2mL/min, the anthocyanin were elution with 4 column volume of ethanol. The resin purified antho-cyanins of blueberry is purplish black powder, color value is 58.96, the recovery rate is 88.53%.