摘要
目的检测TRAIL、c-FLIP在结直肠癌中的表达情况,探讨二者在大肠肿瘤的浸润转移及凋亡调控机制的可能相互关系和作用。方法选取新鲜结直肠癌、配对的癌旁正常组织各85例及转移淋巴结51例,运用Max Vision免疫组化和Western blot蛋白定量检测TRAIL、c-FLIP在各组间的表达,所得结果进行统计学分析。结果 (1)TRAIL在结直肠癌组织中的定位及表达位于细胞膜及细胞浆,而c-FLIP主要位于细胞浆。(2)TRAIL在癌组、转移淋巴结(LNM)组与癌旁正常组的均广泛表达,三者比较差异无统计学意义(P>0.05);癌组中,未/低分化阳性表达与中高分化比较、无LNM组与LNM组比较差异无统计学意义(P>0.05);(3)c-FLIP在癌组阳性表达明显高于正常组(且无一例强染色);转移淋巴结组阳性表达亦明显高于正常组,其强阳性表达88.2%(45/51),与癌组比较差异无统计学意义(P>0.05)。其癌组中未发生淋巴转移阳性表达88.2%(30/34)、淋巴转移100%(51/51)、未/低分化和中高分化分别为96.8%(30/31)、94.4%(51/54),81例阳性表达中强阳性74%(60/81);未/低分化腺癌与中高分化组表达比较差异无统计学意义(P>0.05)。(4)Western blot蛋白定量检测发现,对于TRAIL,呈现出c-FLIP蛋白表达越高,其表达也反应性增高的趋势,TRAIL蛋白的表达与c-FLIP的表达呈相互拮抗性正相关。结论 (1)c-FLIP在结直肠癌组织和转移淋巴结中存在过高表达,在正常组织低表达或不表达,而TRAIL在癌组织和转移淋巴结组织也存在相互拮抗性增高趋势。(2)高表达的c-FLIP可能与TRAIL死亡受体凋亡途径相互作用,对大肠癌的发生进展起到一定的作用。
Objective To investigate the TRAIL, C-Flip expression in colorectal cancer and infiltration transfer mechanism and explore their mutual relations and the role in colorectal tumor invasion and metastasis and apoptosis regulatory mechanism. Methods 85 cases of fresh colorectal cancer tissue specimens, paired adjacent normal colon rectal tissue specimens and 51 cases of metastatic lymph nodes were selected, TRAIL, C-Flip expression were detected by Max Vision immunohistochemistry and Western blot protein quantitative detection in each group, and the results were statistically analyzed. Results （1）The localization and expression of TRAIL in co]orectal carcinoma tissue were cell membrane and cytoplasm, while c-FLIP was mainly located in cytoplasm. （2）TRAIL was Widely expressed in cancer group, lymph node metastasis （LNM） group and adjacent normal group, but there was no significant difference in the three （P 〈 0.05）. In carcinoma group, the positive expression rate of no/low differentiation group compared with medium/high differentiation group, and the positive expression rate of no LNM group compared with LNM group, difference were all not significant （P 〈 0.05）. （3）positive expression rate of c-FLIP in cancer group was significantly higher than that in normal group （without strong staining）; and expression rate of LNM group was obviously higher than that in normal group, the expression rate was 88.2% （45/51）, compared with cancer group, the difference was not significant （P 〉 0.05）. In cancer group, positive expression rate of no LNM group and LNM group were 88.2% （ 30/34 ） and 100% （51/51）, expression rate of no/low differentiation group and medium/high differentiation group were 96.8% （30/31）, 94.4% （51/54）, the difference was not significant（P 〈 0.05）. In 81 cases of positive expression, strong positive expression rate was 74% （60/81）. （4）Western blot protein quantitative detection shows that TRAIL expression had a
出处
《中国医药科学》
2013年第12期10-13,共4页
China Medicine And Pharmacy
