红细胞悬浮液电解质动态变化模型的建立及其初步应用

The establishment and application of dynamic model of red blood cell suspension's electrolyte changing

目的研究建立红细胞悬浮液在保存期内电解质动态变化模型。方法在红细胞保存的24h、3d、5d、7d、14d、21d、28d、35d等8个时间点,分别采样10份,进行K+、Na+、Cl-测定,并对结果进行统计学分析。结果在保存初期内,Na+浓度先下降后上升,但始终比保存初期的Na+浓度要偏低;Cl-浓度在整个保存期内的变化差异无统计学意义;至保存期的第5天,红细胞悬液的K+浓度已经超出正常人参考值范围(3.5～5.5mmol/mL),且随着保存时间的增加,K+浓度也相应增加,但在保存不同的时段,K+上升的速率存在差异。结论红细胞悬浮液电解质动态变化模型的建立有助于指导血液的库存控制,输注保存21d内的红细胞悬浮液有利于减轻患者的钾离子负荷。

Objective To establish the dynamic model of red blood cell suspension＇s electrolyte changing of during the storage time. Methods The samples were collected from red blood cell suspensions after it had been preserved 3 days,5 days,7 days,14 days,21 days,28 days,35 days,and 10 samples were collected at each point time,and them testing K＋ ,Na＋,C1- ,and analysing the results. Results The concentration of Na＋ decreased and then increased in the early stage,but it always been lower than the initial concentration. The concentration of C1- changed less in the preservation period,which were not significant statistically difference. The concentration of K＋ had been beyond the scope of normai reference values（3.5-5.5 mmol/mL）,as the red blood cell suspension were preserved on 3 days,and K＋ increased with the preservation time,but it＇s rate of rise were differed in different periods. Conclusion To establishe the dynamic model of red blood cell suspension＇s electrolyte changing are favorable to control blood inventory,and transfusing the red blood stored in 21days are beneficial for reducing the patient＇s potassium load.