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西瓜防御素基因ClPDF2.1的克隆及表达分析 被引量：2

Cloning and Expression Analysis of Plant Defensin-like Gene (ClPDF2.1) from Watermelon
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摘要 该研究从西瓜中克隆了西瓜防御素基因ClPDF2.1(GenBank登录号为KC481267)。序列分析结果表明,ClPDF2.1基因开放阅读框为225bp,编码75个氨基酸,预测蛋白质分子质量为8.237kD,等电点为9.375。蛋白质结构分析表明,ClPDF2.1蛋白含有防御素特有的8个半胱氨酸结构域。进化分析显示,ClPDF2.1与拟南芥PDF2归为一类,与五彩椒的亲缘关系最近(59%)。荧光定量PCR分析表明,ClPDF2.1在西瓜根、茎、叶器官中都有表达,叶中表达量高于根,茎中表达量最低。ClPDF2.1基因的表达也受到外源植物激素水杨酸、茉莉酸甲酯、乙烯利和西瓜枯萎病菌的诱导上调,表明ClPDF2.1基因通过信号传导途径参与对西瓜枯萎病菌株的防御反应。西瓜防御素基因的克隆及表达分析为其功能鉴定奠定了基础。 In this work, a defensin like cDNA sequence was cloned trom watermelon, ilaliletl （Gengank accession No. KC481267）. Bioinformatics analysis showed that CIPDF2. 1 encodes a putative polypeptide of 75 amino acids with a calculated molecular mass of 8. 237 kD and a theoretical pI of 9. 375. Sequence alignment showed that C1PDF2. 1 had high homology to known PDF proteins from other plant species and contained the conserved eight cysteines motif. Phylogenetic analysis indicated that C1PDF2. 1 belonged to the Arabidopsis PDF2 cluster,and was close to Capsicum annuutn PDF gene with an overall sequence identity of 59%. Real-time PCR analysis revealed that CIPDF2. 1 expressed in all tissues exam ined, with the highest in leaves, second in roots and the lowest in stems. Expression profiles under different treatments such as abscisic acid （SA）,methyl jasmonate （JA）,ethephon （ETH） and Fusarium wilt were compared,and the results revealed that transcriptional level of CIPDF2. 1 was obviously up-regulated. These results suggested that CIPDF2.1 is probably employed the signal pathways to defense against Fu- sarium wilt pathogen.
作者 张曼 羊杏平 王薇薇 徐锦华 刘广 姚协丰 李苹芳
机构地区 江苏省农业科学院蔬菜研究所 江西农业大学农学院
出处 《西北植物学报》 CAS CSCD 北大核心 2013年第5期872-877,共6页 Acta Botanica Boreali-Occidentalia Sinica
基金 国家西甜瓜产业技术体系(CARS-NO.8) 江苏省农业科技自主创新基金[cx(11)1001] 江苏省农业科学院博士后基金(006046511105)
关键词 西瓜 植物防御素 基因克隆 表达分析 watermelon plant defensin-like gene gene cloning expression analysis
分类号 Q786 [生物学—分子生物学]
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参考文献36

  • 1GANZ T. Defensins:antimicrobial peptides of innate immunity[J] Nature Reviews Immunology,2003,3(9):710 720.
  • 2tAZLETT L, WU M. Defensins in innate immunity[J] Cell and Tissue Research, 2011,343 ( 1 ) : 175 - 188.
  • 3THOMMA B P,CAMMUE B P,THEVISSEN K. Plant defensins[J]. Planta,2002,216(2) :193-202. 13.
  • 4D H,J|AN G L,ZHANG Y T,et al. Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusariurn oxysporum[J]. Applied Microbiology and Biotechnology , 2007,74 ( 1 ) : 146- 151.
  • 5DO H M,LEE S C,JUNG H W,et al. Differential expression and in situ localization of a pepper defensin (CADEF1) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsicum annuum [J]. Plant Science, 2004,166(5) : 1 297- 1 305.
  • 6BLOCIt C J, RICHARDSON M. A new family of small (5 kDa) protein inhihitors of insect alphmamylases from seeds of sorghum (ScJr ghum bicolar (L.) Moench. ) have sequence homologies with wheat 7-purothionins[J]. FEBS Letters, 1991,279( 1):101- 104.
  • 7BROEKAERT W F, TERRAS F R,CAMMUE B P,et al. Plant defensins:novel antimicrobiai peptides as components of the host defense system[J]. Plant Physiology, 1995,108(4) : 1 353- 1 358.
  • 8LOBO D S,PEREIRA I B,FRAGEL MADEIRA L,et al. Antifungal Pisum sativum defensin 1 interacts with Neurospora crassa cyclin F related to the celleycle[J].Biochemistry,2007,46(4):987 996.
  • 9SHARMA P, LDNNEBORG A. Isolation and characterization of a cDNA encoding a plant defensin like protein from roots of Norway spruce J]. Plant Molecular Biology, 1996,31 : 707- 712.
  • 10WONG J H, NG T B. Sesquin, a potent defensin-like antimicrobial peptide from ground beans with inhibitory activities toward tumor cells and HIV 1 reverse transcriptase[J].Peptides,2005,26(7)：l 120 1 126.

二级参考文献24

  • 1Rogozhin E A, Oshchepkova Y I, Odintsova T I,et al. No- vel antifungal defensins from Nigella sativa L. seeds [ J ]. Plant Physiol Bioch, 2011,49 : 131 - 137.
  • 2Lay F T, Brugliera F, Anderson M A. Isolation and proper- ties of floral defensins from ornamental tobacco and petu- nia [ J]. Plant Physiol,2003,131 : 1283 - 1293.
  • 3Koike M, Okamoto T,Tsuda S, et al. A novel plant defen- sin-like gene of winter wheat is specifically induced dur- ing cold acclimation [ J ]. Biochem Biophys Res Commun, 2002,298:46 - 53.
  • 4Lay F T,Schirra H J,Scanlon M J,et al. The three-dimen- sional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP [ J]. J Mol Biol, 2003,325 : 175 - 188.
  • 5Carvalho Ade O, Gomes V M. Plant defensins-prospects for the biological functions and biotechnological properties [ J]. Peptides ,2009,30 : 1007 - 1020.
  • 6Bulet P, Stocklin R. Insect antimicrobial peptides: struc- tures,properties and gene regulatioin [ J ]. Protein Pept Lett, 2005,12 : 3 - 11.
  • 7Selsted M E, Ouellette A J. Mammalian defensins in the antimicrobial immune response [ J ]. Nat Immunol,2005, 6:551 -557.
  • 8Mygind P H,Fischer R L,Schnorr K M,et al. Plectasin is a peptide antibiotic with therapeutic potential from a sa- prophytic fungus [ J ]. Nature,2005,437:975 - 980.
  • 9Colilla F J, Rocher A, Mendez E. Gamma-Purothionins : a- mino acid sequence of two polypeptides of a new family of thionins from wheat endosperm [ J ]. FEBS Lett, 1990, 270:191 - 194.
  • 10Mendez E, Moreno A, Colilla F, et al. Primary structure and inhibition of protein synthesis in eukaryoti cell-free system of a novel thionin, gamma-hordothionin, from bar- ley endosperm [ J ]. Eur J Biochem, 1990, 194 : 533 - 539.

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同被引文献23

  • 1Guo L J, Han L J, Yang L Y, et al. Genome and transcriptome analysis of the fungal pathogen Fusazium oxysporum f. sp cubense causing banana vascular wilt disease[J]. PLoS One, 2014, 9 (4): e73 945.
  • 2Vriem K, Cammue B P A, Thevissen K. Antifungal plant dd'emins: mechanisms of action and production [J]. Molecules, 2014, 19 (8): 12280-12303.
  • 3Lay F T, Mills G D, Poon I K H, et al. Dimerization of plant defensin NaD1 enhances its antifungal activity[J]. J Biol Chem, 2012, 287(24) : 19 961-19 972.
  • 4Carvalho A D, Gomes V M. Plant defensins and defensin-like peptides-biological activities and biotechnological applications[J]. Curr Pharm Design, 2011, 17(38): 4270-4293.
  • 5De Coninck B, Cammue B P A, Thevissen IL Modes of antifungal action and in planta functions of plant defensins and defensin- like peptides[J]. Fungal Biol Rev, 2013, 26(4): 109-120.
  • 6Gaspar Y M, McKenna J A, McGinness B S, et al. Field resistance to Fusarium oxysporum and Verticillium dahlioe in transgenic cotton expressing the plant defensin NaDI[J]. J Exp Bot, 2014, 65(6): 1 541-1 550.
  • 7Kong K, Ntui V O, Makabe S, et ol. Transgenic tobacco and tomato plants expressing Wasabi defensin genes driven by root- specific LjNRT2 and AtNRT2.1 promoters confer resistance against Fusarium oxysporum[J]. Plant Biotechnol, 2014, 31(2): 89-96.
  • 8Peng J, Zhang H, Chen F P, et cd. Rapid and quantitative detection of Fusarium oxysporum f. sp cubense race 4 in soil by real-time fluorescence loop-mediated isothermal amplification[J]. J Appl Microbiol, 2014, 117(6): 1 740-1 749.
  • 9Yang L L, Sun LX, Ruan X L, et al. Development of a single-tube duplex real-time fluorescence method for the rapid quantitative detection of Fusarium oxysporum f. sp cubense race 1(Foc 1)and race 4(Foc 4)using TaqMan probes[J]. Crop Prot, 2015, 68: 27-35.
  • 10Zhang X, Zhang H, Pu J J, et al. Development of a real- time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Fusarium oxysporum f. sp cubense tropical race 4 in soil[J]. PLoS One, 2013, 8(12): e82 841.

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西北植物学报
2013年 第5期

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