双氢青蒿素诱导鼻咽癌CNE-2细胞凋亡及其机制

Apoptosis of nasopharyngeal carcinoma cells line CNE-2 induced by dihydroartemisinin and its possible mechanism

目的:探讨双氢青蒿素(DHA)对鼻咽癌CNE-2细胞的生长抑制作用及其机制。方法:以体外培养的CNE-2细胞为研究对象,分别采用CCK-8法观察不同浓度和时间DHA对CNE-2细胞增殖的抑制作用,流式细胞仪Annexin V-FITC法检测CNE-2细胞的凋亡率,caspase-3活性检测法检测不同浓度DHA处理后CNE-2细胞中caspase-3的活性变化。结果:CCK-8法显示,与对照组相比,随DHA浓度的增加,CNE-2细胞的增殖显著抑制(P<0.05);流式细胞仪Annexin V-FITC法,结果显示DHA诱导CNE-2细胞凋亡具有浓度依赖性;caspase-3活性检测法显示,与对照组相比,随DHA浓度的增加,caspase-3的活性显著提高(P<0.05)。结论:DHA能有效抑制CNE-2细胞的增殖并诱导其凋亡,其机制可能是能上调CNE-2细胞中caspase-3的活性。

Objective： Dihydroartemisinin is a traditional anti-malarial drug, a derivative of the artemisinin,it has anti tumor activity of a variety of tumor cells. This study investigated the effect of growth inhibition of naso- pharyngeal carcinoma cells line CNE 2 induced by dihydroartemisinin and its possible mechanism. Method： The effect of DHA on the cell proliferation of CNE-2 was detected by CCK-8 assay with different concentrations and time. The effects of DHA on the cell apoptosis of CNE 2 were detected by Annexin V FITC assay through flow cy tometry and caspase 3 activity assay. Result： CCK-8 experimental results show that CNE-2 cell proliferation was suppressed with DHA treatment, as compared with the control group. DHA could induce marked apoptosis in CNE 2 by apoptosis assay, as compared with the control group, The percentages of apoptotic cells increased along with the increase of DHA concentrations in CNE 2;The activity of caspase 3 was increased following DHA treat ment in a dose-dependent manner;Conclusion： DHA could effectively inhibit proliferation and induce apoptosis of human nasopharyngeal carcinoma cells line CNE 2,the possible mechanism DHA induce apoptosis of CNE 2 cells by upregulating the expression of caspase 3.