miR-26a-1rs7372209基因多态性与冠心病遗传易感的关系

Relationship between miR-26a-1 rs7372209 polymorphism and the genetic susceptibility of coronary artery disease

目的研究miR-26a-1 rs7372209基因型和等位基因频率分布,探讨miR-26a-1 rs7372209基因多态性与华南地区汉族人冠心病遗传易感的关系。方法利用聚合酶链式反应-连接酶检测反应(PCR-LDR)分析技术,对295例冠心病患者和283名对照个体的miR-26a-1 rs7372209(C>T)多态位点进行分型,采用非条件逻辑回归分析统计该多态位点与冠心病易感的相关性。结果 CC、CT、TT基因型在冠心病组中的分布频率分别为54.6%、37.6%和7.8%,在对照组中分别是57.2%、37.5%和5.3%,两组间基因型频率分布差异无统计学意义(χ2=1.554,P=0.460)。携带miR-26a-1 rs7372209 T变异等位基因与冠心病的遗传易感无明显相关(OR=1.15,95%CI=0.88～1.49,P=0.315)。结论 miR-26a-1 rs7372209(C>T)多态位点与华南地区汉族人冠心病易感无相关性。

Objective To investigate the distribution of genotypes and alleles of miR-26a-1 rs7372209 polymorphism, and the corre- lation between this polymorphism and the genetic susceptibility of coronary artery disease （CAD） in the Han nationality of Southern China. Methods The genotypes of the miR-26a-1 rs7372209 （ C 〉 T） polymorphism were determined in 295 CAD patients and 283 control blood donors by polymerase chain reaction-ligase detection reaction （PCR-LDR）. Association between the polymorphism and the risk for CAD was evaluated using unconditional logistic regression analysis. Results The frequency of CC, CT and TT genotypes in the case subjects were 54. 6% , 37.6% and 7.8% respectively and in the control subjects were 57.2% , 37.5% and 5.3%. No significant difference was observed in the frequency of miR-26a-1 genotypes between cases and controls （X2 = 1. 554, P = 0. 460） . Moreover, no significantly increased risk for CAD was observed for those carrying T variant allele （ OR = 1.15,95% CI = 0. 88 - 1.49, P = 0. 315 ）. Conclusions miR-26a-1 rs7372209 （ C 〉 T） polymorphism is not associated with CAD in the Han nationality of Southern China.