摘要
目的:研究白藜芦醇(resveratrol,RES)对辐射损伤的保护作用。方法:建立60Co-γ辐射损伤小鼠模型。200只小鼠随机分为I,II,III,IV 4大组(n=50)。每大组分别又分为正常对照组(n=10)、辐射模型对照组(n=10)和3个RES剂量组(50,100,300 mg/kg,均n=10),经口灌胃30 d后,选择不同剂量60Co-γ照射后继续灌胃至实验结束,并在不同时间分别测定小鼠外周血白细胞数、血清超氧化物歧化酶(superoxide dismutase,SOD)活性、血清溶血素以及骨髓有核细胞数。结果:在不同照射剂量60Co-γ照射和规定的实验条件下,照射后第3天100和300 mg/kgRES剂量组小鼠白细胞计数分别为(1.69±0.82)×109和(1.61±0.51)×109/L,较辐射模型对照组[(0.73±0.69)×109/L]显著上升(分别P<0.05,P<0.01);100和300 mg/kg RES剂量组小鼠骨髓有核细胞数分别为(17.5±4.8)×105和(17.1±4.7)×105/mL,较辐射模型对照组[(7.3±2.2)×105/mL]显著上升(P<0.01);此外,100 mg/kg RES剂量组的小鼠血清SOD活性为(110.41±17.04)U/mL,较辐射模型对照组[(95.80±10.42)U/mL]显著提高(P<0.05);而各剂量RES组中的小鼠血清溶血素差异无统计学意义(均P>0.05)。结论:100和300 mg/kg剂量的RES显示出良好的对抗辐射损伤的作用。
Objective: To explore the anti-radiation pretective effect ofresveratrol (RES). Methods: ^60Co-γ irradiated injury model was established. A total of 200 Kunming mice were randomly divided into 4 groups (50 in each group): Group Ⅰ,Ⅱ,ⅢandⅣ. Each group was sub-divided into 5 groups: a normal control (n=10), an irradiated model control group (n=10) and 3 treatment groups of RES (50, 100, and 300 mg/kg RES treatment groups, 10 in each group). RES was orally administered daily for 30 d in the RES treatment groups and 1% sodium carboxymethylcellulose was orally administered in the normal control and irradiated model group. Thereafter, except the normal control group, the mice in other groups were exposed to different dosages of ^60Co-γ once, and the gavage was continued until the end of different experimental periods. Peripheral leucocytes, nucleated bone marrow cells were counted; superoxide dismutase (SOD) activity and hemolysin in the serum were determined at different time. Results: Under the different dosages of ^60Co-γ irradiation and the provisions of the experimental conditions, the leucocyte count was (1.69±0.82)×10^9 and (1.61±0.51)×10^9/L in the 100 and 300 mg/kg RES treatment groups, which was significantly increased, when compared with the irradiated model control group [(0.73±0.69)×10^9/L] ( P〈0.05, P〈0.01 respectively). The number of nucleated bone marrow cells was (17.3±4.8) and (17.1±4.7)×10^5/mL in the 100 and 300 mg/kg RES treatment groups respectively, which significantly increased when compared with the irradiated model control group [(7.3±2.2)×10^5/mL 1 ( P〈0.01 ). The SOD activity was (110.41±17.04) U/ mL in the 100 mg/kg RES treatment group, which was significantly increased when compared with the irradiated model control group [(95.80±10.42) U/mL ] ( P〈0.05 ). There was no significant difference in the serum hemolysin in all RES treatment groups (all P〉0.05). Conclusion: At 100 and 300 mg/kg, RES has good anti-radiation effect.
出处
《中南大学学报(医学版)》
CAS
CSCD
北大核心
2013年第6期597-601,共5页
Journal of Central South University :Medical Science
基金
湖南省教育厅高等学校研究项目(11C0862)~~
关键词
白藜芦醇
辐射模型
白细胞计数
SOD
血清溶血素
骨髓有核细胞
resveratrol
irradiated model
leucocyte count
superoxide dismutase
serum hemolysin
nucleated bone marrow cell