白藜芦醇对辐射损伤的保护作用

Anti-radiation effect of resveratrol

目的:研究白藜芦醇(resveratrol,RES)对辐射损伤的保护作用。方法:建立60Co-γ辐射损伤小鼠模型。200只小鼠随机分为I,II,III,IV 4大组(n=50)。每大组分别又分为正常对照组(n=10)、辐射模型对照组(n=10)和3个RES剂量组(50,100,300 mg/kg,均n=10),经口灌胃30 d后,选择不同剂量60Co-γ照射后继续灌胃至实验结束,并在不同时间分别测定小鼠外周血白细胞数、血清超氧化物歧化酶(superoxide dismutase,SOD)活性、血清溶血素以及骨髓有核细胞数。结果:在不同照射剂量60Co-γ照射和规定的实验条件下,照射后第3天100和300 mg/kgRES剂量组小鼠白细胞计数分别为(1.69±0.82)×109和(1.61±0.51)×109/L,较辐射模型对照组[(0.73±0.69)×109/L]显著上升(分别P<0.05,P<0.01);100和300 mg/kg RES剂量组小鼠骨髓有核细胞数分别为(17.5±4.8)×105和(17.1±4.7)×105/mL,较辐射模型对照组[(7.3±2.2)×105/mL]显著上升(P<0.01);此外,100 mg/kg RES剂量组的小鼠血清SOD活性为(110.41±17.04)U/mL,较辐射模型对照组[(95.80±10.42)U/mL]显著提高(P<0.05);而各剂量RES组中的小鼠血清溶血素差异无统计学意义(均P>0.05)。结论:100和300 mg/kg剂量的RES显示出良好的对抗辐射损伤的作用。

Objective： To explore the anti-radiation pretective effect ofresveratrol （RES）. Methods： ^60Co-γ irradiated injury model was established. A total of 200 Kunming mice were randomly divided into 4 groups （50 in each group）： Group Ⅰ,Ⅱ,ⅢandⅣ. Each group was sub-divided into 5 groups： a normal control （n=10）, an irradiated model control group （n=10） and 3 treatment groups of RES （50, 100, and 300 mg/kg RES treatment groups, 10 in each group）. RES was orally administered daily for 30 d in the RES treatment groups and 1% sodium carboxymethylcellulose was orally administered in the normal control and irradiated model group. Thereafter, except the normal control group, the mice in other groups were exposed to different dosages of ^60Co-γ once, and the gavage was continued until the end of different experimental periods. Peripheral leucocytes, nucleated bone marrow cells were counted; superoxide dismutase （SOD） activity and hemolysin in the serum were determined at different time. Results： Under the different dosages of ^60Co-γ irradiation and the provisions of the experimental conditions, the leucocyte count was （1.69±0.82）×10^9 and （1.61±0.51）×10^9/L in the 100 and 300 mg/kg RES treatment groups, which was significantly increased, when compared with the irradiated model control group [（0.73±0.69）×10^9/L] （ P〈0.05, P〈0.01 respectively）. The number of nucleated bone marrow cells was （17.3±4.8） and （17.1±4.7）×10^5/mL in the 100 and 300 mg/kg RES treatment groups respectively, which significantly increased when compared with the irradiated model control group [（7.3±2.2）×10^5/mL 1 （ P〈0.01 ）. The SOD activity was （110.41±17.04） U/ mL in the 100 mg/kg RES treatment group, which was significantly increased when compared with the irradiated model control group [（95.80±10.42） U/mL ] （ P〈0.05 ）. There was no significant difference in the serum hemolysin in all RES treatment groups （all P〉0.05）. Conclusion： At 100 and 300 mg/kg, RES has good anti-radiation effect.