半胱氨酸蛋白酶-12表达与细胞色素C释放在脊髓压迫性损伤后少突胶质细胞凋亡中的作用

Role of Caspase-12 expression and cytochrome-C release in oligodendrocyte apoptosis induced by compressed spinal cord injury

目的:探讨半胱氨酸蛋白酶-12(Caspase-12)表达与细胞色素C(cytochrome-C,Cyto-C)释放在脊髓压迫性损伤(compressed spinal cord injury,CSCI)后少突胶质细胞凋亡中的作用。方法:采用自行设计的方法制作脊髓压迫模型,通过原位细胞凋亡染色于压迫后1、3、7 d检测CSCI后少突胶质细胞凋亡;运用Western blot技术从分子水平分别检测与细胞凋亡有关的Caspase-12和Cyto-C的表达。结果:不同组别凋亡的少突胶质细胞数目不全相同(F=30.946,P=0.000),LSD-t法两两比较,1、3、7 d模型组凋亡的少突胶质细胞数目均高于正常组(P=0.000)。与对照组、1 d组、3 d组相比,7 d组凋亡的少突胶质细胞数目最多,组间比较,差异有统计学意义(P=0.000)。不同组别Caspase-12和Cyto-C蛋白表达不全相同(F分别等于1 402.646和326.638,P=0.000),LSD-t法两两比较,1、3、7 d模型组的Caspase-12和Cyto-C蛋白表达均高于正常组(P=0.000),与对照组、1 d组、3 d组相比,7 d组的Caspase-12和Cyto-C蛋白表达水平均最高,组间比较,差异有统计学意义(P=0.000)。结论:CSCI后出现了少突胶质细胞凋亡,其凋亡机制可能与Caspase-12表达增强及Cyto-C释放共同作用有关。

Objective：To evaluate the role of Caspase-12 expression and cytochrome-C （Cyto-C） release in oligodendrocyte apoptosis induced by compressed spinal cord injury（CSCI）. Methods：CSCI model was established with a self-made device. Oligodendrocyte apoptosis was determined by TdT mediated dUTP-biotin nick end labeling staining at 1,3,7 d after compression. Cyto-C release and Caspase-12 expressions were detected by Western blot. Results：Numbers of oligodendrocyte apoptosis in different groups were differ-ent（F=30.946,P=0.000）. Numbers of oligodendrocyte apoptosis in 1,3,7 d groups were more than those in control group according to LSD-t（P=O.O00）. Numbers of oligodendrocyte apoptosis in 7 d group reached the maximum compared with those in control group, 1 d group and 3 d group,with statistically significant differences among groups（P=0.000）. Both expressions of Caspase-12 and releases of Cyto-C in different groups were different（F=l 402.646 and 326.638 respectively,P=0.000）. Expressions of Caspase-12 and releas- es of Cyto-C in 1,3,7 d groups were more than those in control group according to LSD-t（P=0.000）. Expressions of Caspase-12 and releases of Cyto-C in 7 d group reached the maximum compared with those in control group, 1 d group and 3 d group,with statistically significant differences among groups（P=0.000）. Conclusions：Oligodendrocyte apoptosis after CSCI is associated with enhanced expressions of Caspase-12 and releases of Cyto-C.