养血清脑颗粒对缺血/再灌注大鼠脑炎性因子表达和轴突再生的影响

Effects of Yangxueqingnao granule on inflammatory factor expressions and axonal regeneration in rats with focal cerebral ischemia reperfusion

目的:探讨养血清脑颗粒对大鼠局灶性脑缺血/再灌注损伤后皮质核因子-κBp65(nuclear factor-κBp65,NF-κBp65)、白细胞介素-6(interleukin-6,IL-6)、神经微丝蛋白-200(neurofilament protein-200,NF-200)的表达及神经功能的影响。方法:72只健康的成年雄性SD大鼠,随机分为假手术组(S组)、缺血/再灌注组(I/R组),生理盐水对照组(I/R+C组)和养血清脑颗粒治疗组(I/R+YXQ组)。在缺血/再灌注后第2天及第2周,分别采用免疫组织化学法观察NF-κBp65,IL-6,NF-200的表达,West-ern blot法检测缺血脑组织NF-κBp65蛋白的表达,楼梯测试评估大鼠的神经功能。结果:缺血/再灌注后第2天,I/R组大鼠缺血侧皮质NF-κBp65,IL-6表达明显增高(P<0.001),NF-200表达显著降低(P<0.001),同I/R组相比,I/R+YXQ组NF-κBp65,IL-6表达降低(P<0.001);缺血/再灌注后第2周,同I/R组相比,I/R+YXQ组NF-200表达增加(P<0.001),神经功能评分无明显改善(P=0.178)。结论:养血清脑颗粒可能通过减轻局灶性脑缺血/再灌注损伤后皮质神经元的炎症反应,从而促进轴突相关蛋白的表达,改善神经功能。

Objective：To investigate the effects of Yangxueqingnao granule on expressions of nuclear factor-κBp65（NF-κBp65）,interleukin-6（IL-6） and neurofilament protein-200（NF-200） in cortex and neurological function after focal cerebral ischemia reperfusion injury in rats.Methods：Seventy-two SD rats were randomly divided into sham-operated group（S group）,cerebral ischemia/reperfusion group（I/R group）,normal saline control group（I/R＋C group） and Yangxueqingnao granule treated group（I/R＋YXQ group）.Neurological function was measured using Montoya staircase test.Changes in expressions of NF-κBp65 and IL-6 were analyzed by immunohistochemistry and Western blot,respectively.NF-200 immunohistochemical staining was used to evaluate axonal regeneration.Results：At the 2nd d after ischemia reperfusion,expressions of NF-κBp65 and IL-6 in ischemic cortex were significantly increased（P0.001）,while those of NF-200 were significantly decreased in I/R group（P〈0.001）.Expressions of NF-κBp65 and IL-6 were lower in IR＋YXQ group than in I/R group（P〈0.001）.At the 2nd week after ischemia reperfusion,expressions of NF-200 positive cells in I/R＋YXQ group were increased compared with those in the I/R group（P〈0.001） and behavioral tests showed no difference in neurological improvement（P=0.178）.Conclusions：Yangxueqingnao granule can improve neurological function recovery after focal cerebral ischemic reperfusion injury in rats,which may be associated with the inhibition of inflammation reactions.