摘要
目的:探讨在炎症因子与凋亡细胞刺激下对中枢神经系统抗原递呈细胞白细胞介素(interleukin,IL)-27的表达调控。方法:用脂多糖(lipo-polysaccharide,LPS)、干扰素-γ(interferon-γ,IFN-γ)和X射线诱导凋亡细胞对小鼠小胶质细胞和骨髓源树突状细胞刺激。采用实时定量PCR法,检测不同刺激组小胶质细胞和树突状细胞p28基因mRNA的转录水平。Western blot法检测不同刺激组细胞IL-27蛋白表达水平。结果:LPS刺激和LPS与IFN-γ共刺激的小胶质细胞和树突状细胞p28 mRNA表达与空白组相比明显升高(小胶质细胞:PLPS 0.50μg/ml=0.029;PLPS+IFN-γ=4×10-7;树突状细胞:P0.50μg/ml=0.021;PLPS+IFN-γ=8×10-7),凋亡细胞预刺激组p28基因mRNA转录水平明显低于LPS和IFN-γ刺激组,差异有统计学意义(小胶质细胞:PLPS+凋亡细胞=1.2×10-4;PIFN-γ+LPS+凋亡细胞=1×10-7;树突状细胞:PLPS+凋亡细胞=3×10-4;PIFN-γ+LPS+凋亡细胞=2×10-7)。IL-27蛋白表达水平在LPS和IFN-γ刺激组明显增加(小胶质细胞:PLPS 0.25μg/ml=0.022;PLPS+IFN-γ=8×10-7;树突状细胞:PLPS 0.50μg/ml=0.021;PLPS+IFN-γ=2×10-7),在凋亡细胞预刺激组表达下降(小胶质细胞:PLPS+凋亡细胞=9.6×10-5;PIFN-γ+LPS+凋亡细胞=3×10-7;树突状细胞:PLPS+凋亡细胞=0.001;PIFN-γ+LPS+凋亡细胞=1.1×10-5)。结论:中枢抗原递呈细胞在LPS和IFN-γ刺激下IL-27 p28 mRNA和IL-27蛋白表达增加,吞噬凋亡细胞后IL-27 p28 mRNA和IL-27蛋白表达下降。
Objective:To explore how inflammatory factors and apoptotic cells regulate central nervous system(CNS) antigen-presenting cells(APCs) related cytokine-interleukin-27(IL-27) expression in inflammation.Methods:qRT-PCR was used to measure and compare IL-27 p28 mRNA relative expression in mice microglia cells and bone marrow derived dendritic cells(BMDCs),treated with lipopolysaccharide(LPS),interferon-γ(IFN-γ) and X-ray induced apoptotic cells.Western blot was performed to detect IL-27 expression in different stimuli groups.Results:Expressions of IL-27 p28 mRNA and IL-27 were up-regulated when microglia cells and BMDCs being treated with LPS and increased expressions of IL-27 p28 and IL-27 were in relation with LPS dosages.Expressions of IL-27 p28 mRNA and IL-27 were significantly up-regulated when microglia cells and BMDCs being treated with IFN-γ plus LPS(microglia cells:PLPS 0.50 μg/ml=0.029,PLPS+IFN-γ=4×10^-7;BMDCs:P0.50 μg/ml=0.021,PLPS+IFN-γ=8×10^-7).Expressions of IL-27 p28 mRNA and IL-27 were significantly down-regulated when microglia cells and BMDCs being pre-treated with apoptotic cells(microglia cells:PLPS+apoptotic cells=1.2×10-4,PIFN-γ+LPS+apoptotic cells=1×10^-7;BMDCs:PLPS+apoptotic cells=3×10-4,PIFN-γ+LPS+apoptotic cells=2×10^-7).Conclusions:LPS and IFN-γ can up-regulate IL-27 expression in CNS APCs.IL-27 expression is suppressed when CNS APCs phagocytosis of apoptotic cells.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2013年第6期601-607,共7页
Journal of Chongqing Medical University
基金
国家自然科学基金面上资助项目(编号:81271307)