癫痫大鼠模型海马组织突触素-1的表达变化研究

Changes in expressions of synaptophysin-1 in the hippocampus of rats in amygdala-kindling epilepsy model

目的:探讨杏仁核点燃癫痫发生过程中突触素-1(synaptophysin,SYN-1)在海马表达动态变化。方法:建立杏仁核点燃癫痫大鼠模型及左乙拉西坦(levetriacetam,LEV)治疗模型,用real time-PCR测定不同时期SYN的表达量,并测量免疫反应光密度值。结果:SYN-1见于癫痫1周组阳性细胞率最低,表达最少,8周组表达最高,差异均有统计学意义(P<0.05),并且8周组用药前后比较有统计学差异,大鼠在14 d开始出现自发发作。结论:SYN-1与突触形成与重建密切相关,在癫痫组亚组8周组表达上调,提示其可能在慢性期参与了癫痫的维持。

Objective：To establish the amygdala-kindling epilepsy rat model, to measure the expression of synaptophysin-1 （SYN-1） and to explore the pathogenesis of epilepsy. Methods：Totally 140 male Wistar rats were randomly divided into epilepsy group（n=60）, drug treatment group（n=60） and sham operation group（n=20）. Amygdala-kindling epilepsy model and levetiracetam treatment model were established. Expressions of SYN-I at different time points were determined by real-time PCR and absorbance of immunoreaction was detected. R#sults： Expression of SYN-land positive cell rate was the lowest in epilepsy-one-week group and was the highest in epilepsy-eight-week group,with statistical differences among groups（P〈0.05）. There were statistical difference in epilepsy-eight-week group before and after drug treatment;spontaneous seizure was observed in rats at 14 d after drug treatment. Conclusions：SYN-1 is closely related with synaptic formation and reconstruction. Expression of SYN-1 is higher in epilepsy-eight-week group than in sham operation group, suggesting that SYN-1 may be involved in the maintenance of epilepsy at chronic stage.