补充钙剂及维生素D对快速老化小鼠学习记忆能力的影响

Effects of supplementing calcium and vitamin D on learning and memorizing abilities of senescence accelerated mouse P8

目的:观察补充钙剂及维生素D对快速老化小鼠(senescence accelerated mouse,SAM)P8学习记忆能力的影响。方法:3月龄雄性SAMP8,随机分为3组,钙剂(治疗)组、尼莫地平(阳性对照)组、模型(SAMP8)组,每组各9只;另选9只同月龄雄性SAMR1为正常对照组。治疗12周末应用老化度评分比较各组小鼠的老化征象;应用Morris水迷宫检测各组小鼠学习记忆能力。结果:(1)老化度评分显示:钙剂组、尼莫地平组、正常对照组小鼠比模型组小鼠的老化度评分低,差别有统计学意义(P<0.05),钙剂组、尼莫地平组、正常对照组小鼠的老化度评分之间差别无统计学意义(P>0.05);(2)学习记忆能力检测结果提示:Morris水迷宫定位航行实验,钙剂组、尼莫地平组与模型组相比逃避潜伏期明显缩短,差异有统计学意义(P<0.05),与正常对照组相比逃避潜伏期延长,差异有统计学意义(P<0.05),钙剂组与尼莫地平组相比逃避潜伏期无明显差别(P>0.05)。空间探索实验结果提示钙剂组及尼莫地平组穿越平台次数较模型组增加,差异有统计学意义(P<0.05),提示钙剂组及尼莫地平组的记忆保持能力较模型组提高。结论:补充钙剂及维生素D可以减轻SAMP8的老化度,并改善SAMP8的学习记忆能力,效果与应用钙通道阻滞剂尼莫地平接近。提示干预钙代谢紊乱能减轻SAMP8的老化度并改善SAMP8的学习记忆能力,钙代谢紊乱可能是SAMP8学习记忆能力下降的病理生理机制之一。

Objective：To observe the effects of supplementing calcium and vitamin D on learning and memorizing abilities of senescence accelerated mouse P8 （SAMPS）. Methods：Totally 27 three-month-old male SAMP8 were randomly divided into 3 groups： caltrate D group（n=9）,nimodipine group（n=9） and non-treatment SAMP8 group;9 SAMR1 of the same age were used as normal controls（SAMR1 group）. After 12 weeks of treatment,aging status was evaluated for each group with the scores of aging scale;the learning and memory were tested through Morris water maze. Results ： （ 1 ） Based on the aging scale, scores were significantly lower in caltrate D group,nimodipine group and SAMR1 group than in non-treatment SAMP8 group（P〈0.05）;there was no staitical difference among caltrate D group, nimodipine group and SAMR1 group （P〉0.05）. （2）According to Morris water maze,latency in caltrate D group and nimodipine group was significantly shorter compared with that in non-treatment SAMP8 group（P〈0.05）, but was significa- ntly longer compared with that in SAMR1 group（P〈0.05）, there was no statistical difference between caltrate D group and nimodipine group（P〉0.05）. Result of spatial probe test showed that the number of crossing through the platform was increased in caltrate D group and nimodipine group than in non-treatment SAMP8 group,which implied that the ability of memory retention of caltrate D group and nimodipine group was improved significantly compared with that of non-treatment SAMP8 group （P〈0.05）. Conclusions： Supplementing of calcium and vitamin D can alleviate aging and improve learning and memorizing abilities in SAMP8 mice, similar to the effects of applying calcium channel blocker nimodipine. This study suggests that the prevention of calcium metabolic disorder can decrease aging and promote learning and memorizing abilities in SAMP8 mice,indicating calcium metabolic disorder may underlie the pathophysiology of deteriorated learning and memorizing abilities in SAMP8.