微量脑脊液漏快捷定性的可行性研究

Research on the feasibility of easy identification of cerebral spinal fluid leakage with micro sample

目的:通过自身配对研究,探讨对极微量脑脊液快捷定性的可行性。方法:收集44例脑脊液标本,分别运用Olympus au2700全自动生化仪和Johnson onetouch ultral血糖仪试纸法检测其葡萄糖含量,分析两者之间的关系。结果:44例标本通过Johnson onetouch ultral血糖仪试纸法测得的值均显著高于Olympus au2700全自动生化仪检测的糖含量,组间差异具有统计学意义(t=14.14,P<0.05),两者呈正相关(r=0.97),且经一元线性回归方程校正后,简易血糖仪与全自动生化仪检测的数据结果之间的差距无显著性(t=0.01,P>0.05)。Johnson onetouch ultral检测脑脊液葡萄糖含量的精确度为95.7%,平均误差0.12 mmol/L。结论:正确运用Johnson onetouch ultral简易血糖仪,配合现有的血糖试纸,在掌握校正读数值的公式与方法前提下,可以准确检测脑脊液中的葡萄糖含量,藉此对标本进行快捷定性,开发专用检测脑脊液的脑糖仪及脑糖试纸条是切实可行的。

Objective：To discuss the feasibility of easy identification of cerebral spinal fluid leak with micro sample through self control study.Methods：Totally 44 cerebral spinal fluid samples were collected.Olympus au2700 automatic biochemical analyzer and Johnson onetouch ultral blood glucose monitoring system were used to detect their glucose content respectively and to analyze their relationship.Results：There was a positive linear correlation between the outcomes of Olympus au2700 automatic biochemical analyzer and Johnson onetouch ultral glucose monitoring system（r=0.97,P0.05）.Whereas the mean value of the Johnson onetouch ultral group was significantly higher than that of Olympus au2700 group（t=14.14,P0.05）.After a proper calibration of linear regression square（GJP=0.65GJ＋0.21）,there was no significant statistical discrepancy between the two groups（t=0.01,P 0.05）.The average error of using Johnson onetouch ultral blood glucose monitoring system to detect the glucose content of cerebral spinal fluid was 0.12 mmol/L,with an accuracy of 95.7%.Conclusions：Johnson onetouch ultral blood glucose monitoring system,when used properly and followed with correct adjustment,can detect the glucose content of cerebral spinal fluid accurately in less than five seconds with just 0.05 ml sample,making the qualitative diagnosis of cerebral spinal fluid fast and easy and providing a new method in the diagnosis of cerebral spinal fluid leakage.