右主支气管内插管合并胸骨旁开窗术构建单肺通气致兔急性肺损伤动物模型

Establishment of one-lung ventilation induced acute lung injury rabbit model by combining right mainstem bronchial intubation with parasternal fenestration

目的:运用右主支气管内插管合并胸骨旁开窗术构建单肺通气(one-lung ventilation,OLV)致兔急性肺损伤(acute lung injury,ALI)动物模型。方法:24只健康日本大耳白兔随机分为以下4组(n=6):假手术组(S组);双肺通气(two-lung ventilation,TLV)组(T组);TLV加胸骨旁开窗术(parasternal fenestration,PF)组(TPF组)和OLV组(O组)。用支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中中性粒细胞(polymorphonuclear leukocyte,PMN)计数,肺组织髓过氧化物酶(myeloperoxidase,MPO)含量,肺湿/干(wet/dry,W/D)比值和肺组织形态学评分评价肺损伤严重程度。结果:与S组相比,O组、T组与TPF组动物BALF中PMN计数,肺组织MPO活性,肺W/D比值和肺组织形态学评分均明显增高(P<0.05);TPF组和T组动物间上述各项指标差异无统计学意义,但均低于O组(P<0.05)。结论:右主支气管内插管合并双侧PF是一种用于构建OLV致兔ALI的可靠方法。

Objective：To build one-lung ventilation（OLV） induced acute lung injury（ALI） rabbit model by combining right mainstem bronchial intubation with parasternal fenestration. Methods：Totally 24 healthy Japanese white rabbits were randomized into 4 groups （n=6） ：sham operated group （S group ） , two-lung ventilation （TLV） group （T group）,TLV with parasternal fenestration group （TPF group） and one-lung ventilation group（O group）. Lung injury was judged by polymorphonuclear leukocyte （PMN） count in bron-choalveolar lavage fluid （BALF）, myeloperoxidase （MPO） content in pulmonary tissues, lung wet/dry （W/D） ratio and pulmonary histo- logical score. Results：Compared with those in S group, all indicators mentioned above were increased significantly in O group,T group and TF group（P〈0.05）. No difference was observed between T group and TF group, but all indicators in the two groups were lower than those in O group（P〈0.05）. Conclusions：Combining right mainstem bronchial intubation with parasternal fenestration is a simple and reliable method to build rabbit model of OLV induced ALI.