尾加压素Ⅱ转基因家兔制作及其影响因素分析

Creation of urotensin Ⅱ transgenic rabbits by microinjection and analysis of affecting factors

目的分析影响转基因家兔制作的因素,优化转基因动物制作过程,建立适合本实验室的转基因家兔制作方法。方法利用显微注射方法制作转基因家兔,对超数排卵的程序、受精卵的收集、外源基因的显微注射、胚胎移植和术后护理等影响因素都进行了对比研究和探讨。结果优化了转基因家兔制作过程,通过对比发现注射卵泡刺激素方案优于注射孕马血清方案,更易获得较多数量的受精卵。PCR检验结果显示我们成功获得尾加压素Ⅱ转基因家兔。结论通过成功制作尾加压素Ⅱ转基因家兔,建立了适合本实验室的转基因家兔制作方法,同时为研究尾加压素Ⅱ生物学功能和其与疾病的关系提供了有力工具。

Objective To establish a transgenic animal method suitable for our laboratory by optimizing the factors affecting the procedure of transgenic urotesin Ⅱ（U Ⅱ） rabbits creation.Methods U Ⅱ transgenic rabbits were created by microinjection method.We compared the follicle-stimulating hormone（FSH） administration protocol and the pregnant mare serum gonadotropin（PMSG） administration protocol in collection of more zygotes.Results The results showed that the FSH administration protocol was better than the PMSG administration protocol in collection of more zygotes.We successfully established the U Ⅱ transgenic rabbits model by microinjection method.Conclusion We have successfully created UⅡ transgenic rabbits by microinjection method and provided a powerful tool for studies on U Ⅱ biological function and its relationship with diseases.