上皮细胞黏附因子在结直肠癌中的表达与β-连环蛋白的关系及其临床意义

Expression of epithelial cell adhesion molecule and its correlation with β-catenin and its clinical prognostic significance in colorectal cancer

目的探讨上皮细胞黏附因子（EpCAM）在结直肠癌中的表达及其临床意义，探讨其表达与β-连环蛋白（β-catenin）表达的相关性。方法应用免疫组织化学法检测120例结直肠癌组织和30例正常结直肠组织中EpCAM的表达，同时在120例结直肠癌组织中检测β-catenin的表达，结合临床病理特征及随访资料进行统计学分析。结果EpCAM在结直肠癌组织中的高表达率约为60．8％，明显高于正常结直肠组织（6．7％，P〈0．01）；EpCAM在结直肠癌组织中的表达与TMN分期、淋巴结转移、远处转移及复发密切相关（P〈0．05）。8．catenin在结直肠癌的高表达率约为70．0％，其表达与EpCAM的表达呈显著正相关（r=0．518，P〈0．01）。生存分析表明结直肠癌患者的预后与EpCAM的表达、淋巴结转移、远处转移及复发密切相关（P〈0．05）；多因素分析表明EpCAM、TNM分期及淋巴结转移可作为结直肠癌患者的独立预后因素。结论EpCAM在结直肠癌中的表达与β-catenin的表达呈明显正相关。

Objective To investigate the expression and clinical significance of epithelial cell ad- hesion molecule （EpCAM） , and its correlation with β-catenin in colorectal cancer. Methods Immunohis- tochemical method was used to detect the EpCAM expression in 120 cases of colorectal cancer and 30 cases of normal colorectal tissues, and meanwhile detect the 13-catenin expression in 120 cases of colorectal canc- er tissues. The results combined with clinicopathological features and follow-up data were analyzed statisti- cally. Results The rate of high EpCAM expression in colorectal carcinoma tissue （60. 8% ） was obviously higher than that in normal colorectal tissue （ 6. 7% , P 〈 0. 01 ）. The expression of EpCAM in colorectal carcinoma tissue was associated with TMN stages, lymph node metastasis, distant metastasis and recurrence （ P 〈 0. 05 ）. The rate of high β-catenin expression in colorectal carcinoma tissue was 70%, and signifi- cantly correlated with the expression of EpCAM （r = 0. 518, P 〈 0. 01 ）. Survival analysis showed that the prognosis of patients with colorectal cancer was associated with EpCAM expression, lymph node metastasis, distant metastasis and recurrence （P 〈 0. 05 ）. Multiple factor analys.is showed that EpCAM, TNM stages and lymph node metastasis could be used as independent prognostic factors for colorcctal cancer patients. Conclusion The EpCAM expression has a significantly positive correlation with the β-catenin expression in colorectal cancer.