摘要
目的:探索改良高压锅修复法对免疫组化检测ER(EstrogenReceptor,雌激素受体)、Ki-67(细胞增殖活性抗原)两种核抗原表达水平的影响。方法:选取80例乳腺癌组织,分别进行ER和Ki-67两种核抗原在两组不同的修复方法修复后的免疫组化检测。结果:免疫组化采用改良高压锅修复法与传统修复法相比,ER抗原表达水平80例有13例提高,其中有11例由(+)变为(++),2例由(++)变为(+++),(+)和(++)的样本的结果差异有统计学意义(P〈0.05),Ki-67抗原表达水平80例有28例提高,其中20例由(〈25%)变为(1〉25%~50%),有6例由(〉125%~50%)变为(〉150%~75%),有2例由(≥50%~75%)变为(〉175%-100%),总体差异有统计学意义(P〈0.05)。结论:与高压锅传统修复法相比,改良高压锅修复法能提高ER、Ki-67抗原的表达水平,且着色程度更强、节省试剂、方便进行多种pH值修复液同时修复,更加实用。
Objective: To investigate the influence of the improved pressure cooker repair method on the expression level of ER( Estrogen Receptor), Ki-67 (cell proliferative activity)detected by immunohistology. Methods: Eighty cases of breast cancer were selected from the archives of the Pathology Department of our hospital. All specimens were analyzed by immunohistochemieal staining for ER and Ki- 67 which were based on the traditional pressure cooker repair method ( group A) or improved pressure cooker repair method (group B) ,respectively. Results :The expression of ER was enhanced in 13 of 80 cases in group A when compared with that of groupB. Among the 13 cases, 11 cases turned from (+) in group B to (++) in group A, 2 turned from (++) in group B to ( + + + ) in group A. The different expression of ( + ) and ( + + ) in these two groups was statistically significant ( P 〈 0. 05 ). The ex- pression of ki-67 was enhanced in 28 of 80 cases in group A when compared with that of group B. Among the 28 cases , 20 cases turned from 〈25% to ≥25% -50% , 6 turned from ≥25% -50% to ≥50% -75%, 2 turned from ≥50%-75% to t〉75% 100%. The overall expression of Ki-67 in group A was significant different from that of group B (P 〈 0. 05 ). Conclusion:Compared with the traditional pressure cooker repair method, the expression level and intensity of ER, Ki-67 based on improved pressure cooker repair method are improved significantly. Therefore, improved pressure cooker repair method is more practical in daily work.
出处
《肿瘤预防与治疗》
2013年第3期155-158,共4页
Journal of Cancer Control And Treatment