摘要
目的：探索改良高压锅修复法对免疫组化检测ER（EstrogenReceptor，雌激素受体）、Ki-67（细胞增殖活性抗原）两种核抗原表达水平的影响。方法：选取80例乳腺癌组织，分别进行ER和Ki-67两种核抗原在两组不同的修复方法修复后的免疫组化检测。结果：免疫组化采用改良高压锅修复法与传统修复法相比，ER抗原表达水平80例有13例提高，其中有11例由（＋）变为（＋＋），2例由（＋＋）变为（＋＋＋），（＋）和（＋＋）的样本的结果差异有统计学意义（P〈0．05），Ki-67抗原表达水平80例有28例提高，其中20例由（〈25％）变为（1〉25％～50％），有6例由（〉125％～50％）变为（〉150％～75％），有2例由（≥50％～75％）变为（〉175％-100％），总体差异有统计学意义（P〈0．05）。结论：与高压锅传统修复法相比，改良高压锅修复法能提高ER、Ki-67抗原的表达水平，且着色程度更强、节省试剂、方便进行多种pH值修复液同时修复，更加实用。
Objective： To investigate the influence of the improved pressure cooker repair method on the expression level of ER（ Estrogen Receptor）, Ki-67 （cell proliferative activity）detected by immunohistology. Methods： Eighty cases of breast cancer were selected from the archives of the Pathology Department of our hospital. All specimens were analyzed by immunohistochemieal staining for ER and Ki- 67 which were based on the traditional pressure cooker repair method （ group A） or improved pressure cooker repair method （group B） ,respectively. Results ：The expression of ER was enhanced in 13 of 80 cases in group A when compared with that of groupB. Among the 13 cases, 11 cases turned from （＋） in group B to （＋＋） in group A, 2 turned from （＋＋） in group B to （ ＋ ＋ ＋ ） in group A. The different expression of （ ＋ ） and （ ＋ ＋ ） in these two groups was statistically significant （ P 〈 0. 05 ）. The ex- pression of ki-67 was enhanced in 28 of 80 cases in group A when compared with that of group B. Among the 28 cases , 20 cases turned from 〈25% to ≥25% -50% , 6 turned from ≥25% -50% to ≥50% -75%, 2 turned from ≥50%-75% to t〉75% 100%. The overall expression of Ki-67 in group A was significant different from that of group B （P 〈 0. 05 ）. Conclusion：Compared with the traditional pressure cooker repair method, the expression level and intensity of ER, Ki-67 based on improved pressure cooker repair method are improved significantly. Therefore, improved pressure cooker repair method is more practical in daily work.
出处
《肿瘤预防与治疗》
2013年第3期155-158,共4页
Journal of Cancer Control And Treatment
