六味补气胶囊对肺气虚型慢性阻塞性肺疾病大鼠肺组织病理形态及热休克蛋白70表达的影响

Effects of Liuwei Buqi Capsule on the pathological morphology and the expression of HSP70 of lung tissue in COPD rat models with lung-Qi deficiency syndrome

目的探讨六味补气胶囊对肺气虚型慢性阻塞性肺疾病(COPD)大鼠肺组织病理形态学和热休克蛋白70(HSP70蛋白)表达的影响。方法将健康SD大鼠50只随机分为正常对照组、模型组、六味补气胶囊高剂量组、六味补气胶囊低剂量组及脾氨肽组。采用烟熏加脂多糖(LPS)气管滴入法建立COPD肺气虚证实验动物模型,各组灌胃相应的药物或生理盐水。光镜下观察肺组织的病理形态学改变并评分,免疫组化SP法检测肺组织HSP70蛋白的表达。结果模型组肺组织病理评分各项指标明显高于正常对照组(P<0.01),六味补气胶囊高剂量组与脾氨肽组肺组织支气管黏膜上皮病变、支气管壁炎细胞浸润2项指标病理评分显著低于模型组(P<0.05或P<0.01),六味补气胶囊低剂量组与模型组比较差异无统计意义(P>0.05)。与正常对照组比较,模型组HSP70蛋白在肺组织支气管上皮细胞、肺泡上皮细胞和炎症细胞中高表达(P<0.01);六味补气胶囊高剂量组与脾氨肽组HSP70蛋白表达显著低于模型组,2组比较差异有统计意义(P<0.05);六味补气胶囊低剂量组HSP70蛋白表达与模型组比较差异无统计意义(P>0.05)。结论六味补气胶囊能够减轻COPD肺气虚证大鼠肺组织的损伤程度及炎症反应,降低HSP70蛋白的表达。

Objective To explore the effects of Liuwei Buqi Capsule （LBC） on the pathological morphology changes and the expression of heat shock protein 70 （HSP70） of lung tissue in chronic obstructive pulmonary disease （COPD） rat models with lung-Qi deficiency syndrome. Methods Fifty SD rats were randomly divided into ： normal control group （ N）, model group （ M ） , high dose LBC group （LH） , low dose LBC group （LL） and spleen aminopeptide（SA） group. The rat models of COPD with lung-Qi deficiency syndrome were established by intratrachealinstillation of lipopolysaccharide（LPS） twice and exposed to cigarette smoke. HE staining was used to ob- serve the pathological morphology changes of lung tissue and score, expressions of HSP70 in lung tissue were detec- ted by immunohistochemical method. Results The pathological score increased significantly in M group compared with those in N group（P〈0.01 ）. The pathological score of LH group and SA group were significantly lower than M group（P〈0.05 or P〈0.01 ）. The LL group had no significant difference compared with the M group（ P〉0.05 ）. The expression of HSP70 in bronchia epithelial cells of lung tissue, part of alveolar epithelial cells and inflammatory cells increased significantly in M group compared with those in N group（P〈0.01 ）, the expression of HSP70 decreased significantly in LH and SA group compared with those in M group（ P〈0.05 ） , the expression of HSP70 was no statistical difference in LL group compared with those in M group（P〉0.05）. Conclusion LBC could reduce the degree of injury, inflammatory responses and expression of HSP70 in the lung tissues of COPD rat models with lung-Qi deficiency syndrome.