相同产地黄芩HPLC指纹图谱研究

STUDY ON THE FINGERPRINTS OF SCUTELLARIA BAICALENSIS FROM SAME PRODUCTION PLACES

目的:建立相同产地黄芩药材的HPLC指纹图谱,以探讨相同产地黄芩药材的个体差异。方法:采用HPLC法测定20个采自内蒙古赤峰的黄芩样品的HPLC指纹图谱,色谱条件:Agilent TC-C18柱(4.6mm×250mm,5μm),乙腈与0.1%冰醋酸溶液进行梯度洗脱,检测波长276nm,流速1ml/min,柱温25℃。运用中药色谱指纹图谱相似度评价系统分析各个样品的相似度。结果:20个黄芩药材样品的HPLC指纹图谱主要有6个共有峰,样品相似度均达到0.9以上,但共有峰相对面积有一定的差异。结论:相同产地黄芩HPLC指纹图谱分析方法可靠,可为以后研究不同产地黄芩药材HPLC指纹图谱奠定基础。

Objeelive：To study the difference of Scutellaria baicalensis from same production places by establishing HPLC fingerprints of Scutellaria baicalensis. Methods：HPLC fingerprints of 20 Scutellaria baicalensis samples from Neimenggu Chifeng were analyzed by HPLC. The chromatographic condition： Agilent TC-CI8 Column （4.6mm×250rnm, 5μm）, mobile phase was acetonilrile and 0.1% glacial acetic acid in gradient elution pmgrarn, detecting wave length 276nrn, flow rate 1.0 ml/min, and column temperature 25℃. The similarity of each sample was analyzed by chromatographic fingerprint similarity evaluation system. Results：20 Scutellaria baicalensis samples had 6 common peaks. The similarity of 20 samples were all higher than 0.9, but there had some differences between the relative area of the common peaks. Conclusions： HPLC fingerprints of Scutellaria baicalensis from same production places will provide a reference standard for the study of Scutellaria baicalensis from different production places.