黄芪发酵颗粒对气虚冻伤模型大鼠免疫系统影响的研究

Research of the Impacts of Astragalus Fermentation Granules on the Immune System for the Rat Models of Qi Deficiency and Frozen Injury

目的为研究证实黄芪发酵颗粒对气虚冻伤模型大鼠免疫系统的影响。方法从黄芪补气升阳,益卫固表的功效着手选用与之相对应的气虚冻伤大鼠模型作为研究对象,选用大鼠白细胞介素-1(IL-1)、白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、肿瘤坏死因子β(TNF-β)等与免疫系统密切相关的指标作为检测对象,探讨三种剂量的黄芪发酵颗粒及黄芪药材颗粒对气虚冻伤大鼠模型的免疫系统影响。结果三种剂量的黄芪发酵颗粒对气虚冻伤大鼠模型的免疫系统均有影响,且高剂量黄芪发酵颗粒组对大鼠IL-1、IL-2、IL-6、TNF-β的影响更为明显,与模型组比较,差异有统计学意义(P<0.05)。结论黄芪发酵颗粒对气虚冻伤模型大鼠的IL-1、IL-2、IL-6、TNF-β均有影响,其中对IL-2和IL-6的影响较为明显,提示其仍具有补气升阳,益卫固表的功效。

Objective To verify the impacts of astragalus fermentation granules on the immune system for the rat models of qi deficiency and frozen injury. Methods Astragalus acts on tonifying qi, rising up yang, benefiting the defensive qi and strengthening the exterior. The rat models of qi deficiency and frozen injury were selected as the subjects. The indicators closely related to the immune system such as interleukin - 1 （ IL - 1 ）, interleukin - 2 （ IL - 2 ）, interleukin - 6 （ IL - 6 ） and tumor necrosis factor 13 （ TNF - [3 ） were taken as the determination objects. Astragalus fermentation granules and astragalus material granules of three doses were used to observe the impacts on the immune system for the rat models of qi deficiency and frozen injury. Results Astragalus fermentation granules of three doses influenced the immune system of the rat models of qi deficiency and frozen injury and the levels of IL - 1, IL - 2, IL - 6 and TNF - [3 were impacted much more apparently for the rats in the high - dose group, presenting the statistical significant difference as compared with the model group （ P 〈 0.05 ）. Conclusion Astragalus fermentation granules impact the levels of IL - 1, IL -2, IL -6 and TNF - 13 for the rat models of qi deficiency and frozen injury,in which, the influence on IL -2 and IL -6 is more apparent, suggesting that this drug still acts on tonifying qi, rising up yang, benefiting the defensive ai and strenathening the exterior.