质量分数权重系数法表征银杏内酯组分溶解性及表观油水分配系数的探讨

Study on solubility of ginkgolide components with mass fraction weight coefficient method

该研究采用HPLC-ELSD测定银杏内酯A和银杏内酯B在水和不同pH缓冲溶液中的平衡溶解度及其在正辛醇-水/缓冲盐溶液中的表观油水分配系数,进一步通过质量分数权重系数法,整合表征银杏内酯组分整体的平衡溶解度和整体的油水分配系数。研究中对直接加和法和质量分数权重系数整合法表征银杏内酯组分性质值进行了比较,发现质量分数权重系数整合表征中药组分性质更为科学合理,通过该尝试性研究可以为中药组分的性质表征提供一定的研究思路,同时为中药组分制剂研究提供依据。

In this study, the HPLC-ELSD method was adopted to detect the equilibrium solubility of B and A in water and dif- ferent pH buffer solution, as well as their apparent oil-water partition coefficients in the noctanol-water/buffer solution. Furthermore, the mass fraction weight coefficient method was adopted to express the integrated equilibrium solubility and oil-water distribution coeffi- cient of ginkgolide components. The direct addition method and the mass fraction weight coefficient method were compared in calculat- ing the property value of ginkgolide components, showing that the mass fraction weight coefficient method was more scientific and rea- sonable. The tentative study provided ideas to property characterization of traditional Chinese medicine components, as well as basis for studies on preparations of traditional Chinese medicine components.