HPLC测定野菊花药材中8种黄酮和有机酸的含量

Simultaneous determination of eight active components in Chrysanthemum indicum by HPLC

建立同时测定野菊花药材中绿原酸、咖啡酸、1,3-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、木犀草素-7-O-β-D-葡萄糖苷、3,4-二咖啡酰奎宁酸、蒙花苷及木犀草素等8种成分含量的方法。采用HPLC,Shim pack VP-ODS色谱柱(4.6 mm×250 mm,5μm);以甲醇(A)-0.3%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL.min-1;检测波长326 nm;柱温35℃。8种成分在40 min内均达到基线分离,线性关系良好(r>0.999 5,n=7);平均回收率均在97.03%～102.3%(RSD<2.0%,n=6)。结果表明该方法操作简单,准确性和重现性良好,为野菊花药材的质量控制提供科学依据。

This study is aimed to establish a high-performance liquid chromatography （HPLC） method for simultaneous deter- mination of chlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, 3,4-dicaf- feoylquinic acid, linarin and luteolin in Chrysanthemum indicum. The separation was carried out on a Shim pack VP-ODS（4.6 mm × 250 mm, 5 μm） column eluting with mobile phases of methanol （A） and water containing 0.3% phosphoric acid （B） in gradient mode （0-9 min, 85%-80%B; 9-12 min, 80%-70%B; 12-15min, 70%-65%B; 15-20 min, 65%-60%B; 20-23 min, 60%-55%B; 23-29 rain, 55% -54.4% B ; 29-32 min, 54.4% -45% B ; 32-37 min, 45% -5% B ; 37-45 min, 5%-85% B） at the flow rate of 1.0 mL .min-1. The column temperature was 35 ℃ and the detection wavelength was set at 326 nm. The good separation of chlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, 3,5-dicaffeoylqninic acid, luteolin-7-O-β-D-glucoside, 3,4-dicaffeoylquinic acid, linarin and luteolin was achieved within 40 rain. Calibration curves of the eight effective components showed good linear relationship（r 〉 0. 999 5, n = 7 ）. The average recoveries were within 97.03 % -102.3 % （ RSD 〈 2.0%, n = 6）. The method is simple, accurate and repeatable and can be used for the quality control of Ch. indicum.