白头翁皂苷调控RA模型大鼠FLS SFRP2表达

Effect of pulchinenoside in regulating FLS SFRP2 expression of RA model rats

目的:研究中药白头翁主要活性成分白头翁皂苷(pulchinenoside,PULC)对类风湿性关节炎(rheumatoid arthritis,RA)模型大鼠成纤维细胞样滑膜细胞(fibroblast-like synoviocytes,FLS)SFRP2表达的调控。方法:采用大鼠关节炎评分法和足爪肿胀评分法评价PULC对RA模型大鼠的治疗作用,MTT法检测PULC灌胃治疗后对FLS增殖抑制作用。RT-PCR,Westernblotting检测PULC灌胃治疗对FLS SFRP2基因表达的调控作用,对Wnt通路关键基因β-catenin,Wnt通路下游效应基因C-myc的调控作用。结果:RA模型大鼠FLS中SFRP2基因表达明显下调,经PULC灌胃治疗后,RA模型大鼠FLS中SFRP2表达上调,β-catenin和C-myc基因表达明显下调。结论:PULC通过对Wnt通路的调控抑制RA模型大鼠滑膜异常增殖。

Objective： To study the effect of pulchinenoside （PULC） in modulating SFRP2 expression in fibroblast-like syno- viocytes （FLS） of rheumatoid arthritis （RA） model rats. Method： The effect of PULC in treating RA rats was evaluated by rat arthri- tis score and paw swelling score. The inhibitory effect of PULC on FLS proliferation was detected by MTT reagent. The effects of PULC gavage treatment in modulating gene expression of FLS SFRP2, critical gene β-catenin of Wnt pathway and downstream effector genes C-myc of of Wnt pathway were detected by RT-PCR and Western blotting. Result： PULC had a significant effect in treating RA rats and that SFRP2 expression was down-regulated in FLS. After PULC gavage treatment, FLS SFRP2 expression was obviously up-regula- ted, whereas β-catenin and C-myc gene expressions were significantly down-regulated. Conclusion： PULC can inhibit abnormal prolif- eration of synovial membrane by modulating Wnt pathway of RA rats.