摘要
目的为建立栀子苷的免疫学分析方法,首先合成栀子苷的人工完全抗原。方法采用高碘酸钠氧化法分别合成栀子苷免疫抗原(GEN-BSA)和包被抗原(GEN-PLL)。结果优化了栀子苷抗原的合成条件,经紫外光谱检测栀子苷成功与BSA/PLL偶联,偶联比可达到11∶1,免疫小鼠后血清抗体效价达到1∶12 800。结论成功合成栀子苷完全抗原,可用于下一步单克隆抗体的制备。
Objective To establish a immunoassay for analyzing geniposide (GEN), and synthesize the artificial complete antigen of GEN. Methods The immunizing antigen (GEN-BSA) and coating antigen (GEN-PLL) were synthesized respectively by applying sodium periodate oxidation method. Results The synthesis conditions of GEN antigen was optimized. After the detection of ultraviolet spectrum, GEN was successfully conjugated with BSA/PLL and the conjugation ratio was 11: 1. The serum antigen titer had reached to 1:12 800 in immunized mice. Conclusion The complete antigen of GEN has been successfully synthesized, which can be used further for preparing monoclonal antibody.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2013年第6期387-392,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.30973709
81274043)
教育部回国人员科研启动基金项目
重大新药创制科技专项(No.20102X09502-002)
关键词
栀子苷
人工抗原
高碘酸钠氧化法
geniposide
artificial antigen
sodium periodate oxidation method