摘要
目的为建立栀子苷的免疫学分析方法,首先合成栀子苷的人工完全抗原。方法采用高碘酸钠氧化法分别合成栀子苷免疫抗原(GEN-BSA)和包被抗原(GEN-PLL)。结果优化了栀子苷抗原的合成条件,经紫外光谱检测栀子苷成功与BSA/PLL偶联,偶联比可达到11∶1,免疫小鼠后血清抗体效价达到1∶12 800。结论成功合成栀子苷完全抗原,可用于下一步单克隆抗体的制备。
Objective To establish a immunoassay for analyzing geniposide （GEN）, and synthesize the artificial complete antigen of GEN. Methods The immunizing antigen （GEN-BSA） and coating antigen （GEN-PLL） were synthesized respectively by applying sodium periodate oxidation method. Results The synthesis conditions of GEN antigen was optimized. After the detection of ultraviolet spectrum, GEN was successfully conjugated with BSA/PLL and the conjugation ratio was 11： 1. The serum antigen titer had reached to 1：12 800 in immunized mice. Conclusion The complete antigen of GEN has been successfully synthesized, which can be used further for preparing monoclonal antibody.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2013年第6期387-392,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.30973709
81274043)
教育部回国人员科研启动基金项目
重大新药创制科技专项(No.20102X09502-002)
关键词
栀子苷
人工抗原
高碘酸钠氧化法
geniposide
artificial antigen
sodium periodate oxidation method
