参青方对溃疡性结肠炎大鼠黏膜地址素细胞黏附分子表达的影响

Influence of Shenqing Fang on expression of MAdCAM-1 in rats with ulcerative colitis

目的探讨参青方治疗溃疡性结肠炎的作用机制。方法采用三硝基苯磺酸灌肠诱导建立大鼠溃疡性结肠炎模型。SD大鼠60只,随机分为正常组、模型Ⅰ组、模型Ⅱ组、美沙拉嗪组、参青方低剂量组、参青方高剂量组共6组,每组10只。运用免疫组织化学染色、RT-PCR、Western-blot实验技术检测模型大鼠结肠黏膜地址素细胞黏附分子(MAdCAM-1)的表达。结果正常组大鼠结肠黏膜MAdCAM-1可见少量表达,模型组MAdCAM-1蛋白表达与基因转录量均高于正常组,差异具有统计学意义(P<0.01);参青方高、低剂量组及美沙拉嗪组与模型Ⅱ组比较,MAdCAM-1的表达均明显下调,差异具有统计学意义(P<0.01),且以参青方高剂量组降低趋势更为明显。结论参青方具有治疗大鼠溃疡性结肠炎的作用,其机制与降低MAdCAM-1在结肠黏膜的表达有关。

Objective To discuss the mechanism of Shenqing Fang in treatment on ulcerative colitis （UC）. Methods The rat model of UC was established by the enema of TNBS in rats. SD rats （ n = 60） were randomly divided into normal group, model group 1, model group 2, mesalazine group, low-dose Shenqing Fang （low-dose group） and high-dose Shenqing Fang （high-dose group, each n = 10）. The expression of colonic mucosal addressin cell adhesion molecule-1 （ MAdCAM-1 ） was detected by applying immnohitological staining, reverse transcription polymerase chain reaction （RT-PCR） and Western-blot test. Results The expression of MAdCAM-1 was lower in normal group, and protein expression and gene transcription were higher in model groups than those in normal group （P 〈 0.01 ）. The expression of MAdCAM-1 was significantly down-regulated in high-dose group, low-dose group and mesalazine group compared with model group 2 （ P 〈 0.01 ） , and the decreasing trend was more significant in high-dose group. Conclusion Shenqing Fang has therapeutic effect on UC, and the mechanism may be related to that it can reduce the expression of MAdCAM-1.