帕瑞昔布钠预先干预对经前期综合征模型大鼠的作用

The effects of pre 4ntervention parecoxib sodium on premenstrual syndrome model rats

目的研究帕瑞昔布钠预先干预对经前期综合征（premenstrual syndrome，PMS）模型大鼠的作用。方法36只Wiser大鼠依阴道涂片法确定大鼠动情周期，分别将处于动情后期的大鼠采用完全随机分组方法分为：正常组（c组），模型组（M组），模型＋帕瑞昔布钠组（A组）。采用慢性束缚应激法复制PMS模型，持续7d。造模第5天。7天，A组给予帕瑞昔布钠肌注0．72ms／ks。造模成功后，于第8天处死大鼠。免疫组化检测大鼠子宫内膜组织环氧化酶-2（cycloxygenase-2，COX-2）、前列腺素E2（prostaglandin E2，PGE2）的表达，Westernblot法检测大鼠大脑海马组织COX-2、PGE2的表达。结果①M组和A组大鼠子宫内膜COX-2表达高于C组，差异有统计学意义（P〈0．01）；与M组（0．46±0．11）比较，A组（0．31±0．12）子宫内膜COX-2表达显著降低32％（P〈0．05o②M组和A组子宫内膜PGE2表达较c组显著增加（P〈0．01）；与M组（0．49±0．08）比较，A组（0．39±0．09）子宫内膜PGE2表达显著降低18．9％（P〈0．05）.③M组和A组大鼠海马组织COX-2表达高于C组，差异有统计学意义（P〈0．01）；与M组（0．91±0．03）比较，A组（0．72±0．05）大鼠海马组织COX-2表达显著降低20．8％（P〈0．05）。④M组和A组大鼠海马组织PGE2表达较c组显著增加（P〈0．01）；与M组（1．13±0．03）比较，A组（0．92±0．06）大鼠海马PGE2表达显著降低18％（P〈0．05）.结论帕瑞昔布钠预先干预能降低PMS模型大鼠子宫内膜及大脑海马部位COX-2、PGE2的表达。

Objective To investigate the effects of pre-intervention parecoxib sodium on premenstrual syndrome （PMS） model rats. Methods 36 rats were randomly divided into 3 groups according to the results of vaginal secretion smear, the normal group （group C）, the model group （group M） , and the pre-intervention parecoxib sodium group （group A）. PMS models were prepared by chronic restraint stress, which persist for 7 d. Group A was pre-administered parecoxib sodium 0.72 mg/kg from the fifth day to the seventh day. All the rats were sacrificed at the eighth day. Then we applied immunohistochemical to detect the cycloxygenase-2 （COX-2） and prostaglandin E2 （PGE2） expression on rat endometrium, and western blot to detect the COX-2 and PGE2 expression on rat hippocampus. Results （1） COX-2 expression on rat endometrium of group M and group A was significantly higher than group C（P〈0.01 ）. COX-2 expression on the endometrium of group A（0.31±0.12） was 32% lower than group M（0.46±0.11 ） （P〈0.05）. （2） PGE2 expression on rat endometrium of group M and group A was significantly higher than group C （P〈0.01）. PGE2 expression on endometrium of group A （0.39±0.09） was 18.9% lower than group M （0.49±0.08）（P〈0.05）. （3） COX-2 expression on hippocampus of group M and group A was significantly higher than group C （P〈0.01）. COX-2 expression on hiptx）campus of group A （0.72±0.05） was 20.8% lower than group M （0.91±0.03）（P〈0.05）. （4） PGE2 expression on hippoeampus of group M and group A was significantly higher than group C （P〈0.01）, COX-2 expression on hippocampus of group A （0.92±0.06） was 18% lower than group M （1.13±0.03）（P〈0.05）. Conclusions Parecoxib sodium pre-intervention could decrease COX-2 and PGE2 expression on endometrium and hippocampus in premenstrual syndrome model rats.