罗格列酮对重症急性胰腺炎胰岛β细胞作用的研究

Effects of rosiglitazone on pancreatic β cells in a model of severe acute pancreatitis

目的观察罗格列酮对重症急性胰腺炎（severe acute pancreatitis，SAP）大鼠胰岛β细胞的作用。方法雄『生Wistar大鼠36只，按数字表法随机分为假手术组（SO组）、重症急性胰腺炎组（SAP组）和罗格列酮干预组（ROSI组），每组12只。胆胰管逆行注射5％牛磺胆酸钠制备SAP模型。sO组和SAP组造模前30min股静脉注射10％二甲基亚砜（dimethyl sulfoxide，DMSO）（0．2ml／100g）；ROSI组则注射10％DMSO溶解的罗格列酮（6mg/kg）。术后12h剖杀大鼠，检测血清淀粉酶（amylase，AMY）水平；取胰头部胰腺组织行病理学检查，并行胰岛素原位染色检测；电镜观察胰岛B细胞超微结构的变化。结果SAP组造模成功后，血清AMY、胰腺组织病理评分明显升高，与SO组比较差异有统计学意义（P〈0．05）；ROSI干预后血清AMY和胰腺组织病理评分较SAP组明显降低，2组比较差异有统计学意义（P〈0．05）。电镜观察ROSI干预后胰岛β细胞形态、结构较SAP组完整，同时其胰岛素原位染色较SAP组深，但低于SO组胰岛素染色的表达。结论罗格列酮在SAP过程中对胰腺β细胞形态和分泌功能具有保护作用。

Objective To explore the effects of rosiglitazone on pancreatic β cells in a rat model of severe a- cute pancreatitis （SAP）. Methods 36 male Wistar rats were randomly divided into sham operation group （SO group）, SAP group, and rosiglitazone pretreatment group（ ROSI group）. SAP model was induced by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct. SO and SAP group were injected 10% dimethyl sulfoxide partes aequales（DMSO） （0. 2 ml/100 g）by femoral vein 30 rains piror to the surgery. ROSI group were injected rosiglitazone （6 mg/kg）instead of 10% DMSO. Rats were killed 12 h after surgery and the level of serum amylase was detected. Pancreas tissues were taken for pathological examination under light microscope and insulin in situ staining was done. The uhrastructure of β cells was observed with transmission electron microscope. Results Amylase level and pathologic score were increased significantly in SAP group than in SO group （ P 〈 0. 05）. Compared with SAP group, amylase level and pathologic score reduced significantly for rats in ROSI group （P 〈 0. 05）. Compared with SAP group, the morphological structure of β cells in ROSI group was more completed and insulin in situ staining was darker observed by electronic microscopy. Conclusion ROSI exerts protective effect on morphology and endocrine function of pancreatic cells in rats with severe acute pancreatitis.