基于微透析的组织液中葡萄糖浓度连续检测方法

A Method for Continuous Glucose Monitoring in Interstitial Fluid Based on Microdialysis

基于微创血糖检测中需要对组织液中葡萄糖浓度进行校准的要求,提出了一种利用微透析连续检测组织液中葡萄糖浓度的方法,研究了灌流速度、被测液浓度和温度等条件对回收率的影响.实验结果表明,灌流速度从0.3,μL/min上升到3.0,μL/min时,回收率下降71.7%;组织液浓度变化对回收率没有明显影响,温度从25,℃上升到58,℃时,回收率则上升34.6%.此外,提出了一种模拟体内葡萄糖浓度连续变化的方法,并建立了实验系统,验证了微透析方法在葡萄糖浓度连续变化条件下用于组织液葡萄糖检测的可行性.为进一步开展动物实验提供了理论基础和实验依据.

A method based on microdialysis was presented to monitor glucose concentration in interstitial fluid to meet the requirement that the glucose concentration in interstitial fluid should be calibrated in minimally invasive blood glucose monitoring, and the influences of perfusion rate, concentration and temperature on the recovery rate of microdialysis were studied. The results led to the conclusion that the recovery rate fell by 71.7% when perfusion rate increased from 0.3 μL/min to 3.0 μL/min, different concentrations of interstitial fluid contributed little to the recovery rate, and the rise of temperature from 25 ℃ to 58 ℃ caused the recovery rate to increase by 34.6%. In addition, a method for simulating the rapid changes in glucose concentration in vivo was proposed, and the experimental system was established to verify the feasibility of continuous measurement of glucose level in interstitial fluid by microdialy- sis. All the work laid a theoretic foundation and experimental guidance for animal clinical trials in the future.