STAT3在小细胞肺癌中的表达及对血管生成的影响

STAT3 Expression in Small Cell Lung Cancer and Angiogenesis

目的探讨信号传导和转录激活因子(STAT3)及其磷酸化形式p-STAT3在小细胞肺癌(SCLCs)中的表达及STAT3与小细胞肺癌血管生成的关系。方法收集64例小细胞肺癌患者的肺癌组织及癌旁正常肺组织,采用WesternBlot检测组织中STAT3和p-STAT3的蛋白表达,采用RT-PCR法检测组织中STAT3的mRNA表达,同时观察组织中微血管密度(MVD)的水平。结果与正常肺组织相比,小细胞肺癌组织中STAT3和p-STAT3的蛋白表达以及STAT3的mRNA表达水平均明显升高(P<0.05)。肺癌组织STAT3和p-STAT3的表达水平与患者的总生存率、淋巴结转移,临床分期和肿瘤大小正相关(P<0.05)。STAT3的表达与肺癌组织中MVD的水平成正相关。结论 STAT3及其磷酸化形式在小细胞肺癌的表达升高,且其与小细胞肺癌患者的临床病理特征具有相关性,可能是临床治疗的新靶点。

Objective To investigate expressions of signal transduction and activator of transcription factor （STAT3） and its phosphorylated form （p-STAT3） in small cell lung cancer （SCLCs）, and the relations with vascular generated. Methods Lung cancer group was lung tissue of 64 patients with small cell lung cancer and its surrounding normal lung tissue as control group. Western Blot was used to detect STAT3 and p-STAT3 protein expression and RT-PCR. was used to analysis STAT3 mRNA expression, while observing the level of both tissue microvascular densities （MVD）. Results Compared with normal lung tis-sue, STAT3 and p-STAT3 mRNA and protein levels in Lung cancer group were significantly increased （P〈 0.05）. STAT3 and p-STAT3 expressions in the lung tissue were positively related to survival, lymph node metastasis, clinical stage, and minor size （P〈 0.05）. STAT3 expression in lung tissue was positively reLated to MVD levels. Conclusion STAT3 plays an important role in the occurrence and development of small cell lung cancer, and STAT3 maybe a new target for small cell lung cancer.