类群特异性PCR引物设计与评估

Design and evaluation of group-targeted PCR primers

本文以真核藻类18SrDNA类群特异性PCR引物的设计与评估为例,详细介绍了如何利用Primrose等一系列程序设计类群特异性PCR引物并评估其敏感性与特异性,并对这一方法的优势与应用类群特异性PCR引物进行群落多样性分析需要注意的问题进行了讨论。

We take the design and evaluation of eukaryotie algae-targeted 18S rDNA PCR primers as an example, to give a step-by-step illustration of how to design and assess group-targeted PCR primers using Primrose and other programs. Then we summarize the advantages of the design ＆ evaluation pipeline by comparing the amplification ef- ficiency of newly designed and previously reported 18S rDNA universal primers. Cautions are also emphasized on u- sing PCR-based strategies to assess community diversity.